Table 1. Transduction levels in... | American Society of Hematology

Table 1.

Transduction levels in progenitor cells from hosts engrafted with WT or TGF-β1^−/− virus–infected cells

Time after transplantation	WT transplant			TGF-β1^−/− transplant
Time after transplantation	Mouse	TPO/actin	% positive	Mouse	TPO/actin	% positive
Week 6	WT1	10/28	36	KO9	7/21	33
	WT3	21/30	70	KO10	2/25	8
	WT4	27/30	90	KO12	10/17	59
	WT11	9/30	30
Week 16	WT2	7/26	26	KO1	2/28	7
	WT5	5/30	16	KO7	0/30	0
	WT8	12/28	43	KO8	27/28	96
	W14	4/30	13	KO11	1/29	3
				KO14	19/30	63

Time after transplantation	WT transplant			TGF-β1^−/− transplant
Time after transplantation	Mouse	TPO/actin	% positive	Mouse	TPO/actin	% positive
Week 6	WT1	10/28	36	KO9	7/21	33
	WT3	21/30	70	KO10	2/25	8
	WT4	27/30	90	KO12	10/17	59
	WT11	9/30	30
Week 16	WT2	7/26	26	KO1	2/28	7
	WT5	5/30	16	KO7	0/30	0
	WT8	12/28	43	KO8	27/28	96
	W14	4/30	13	KO11	1/29	3
				KO14	19/30	63

Irradiated WT hosts were engrafted with virus-infected marrow cells from WT or TGF-β1^−/− (knock-out [KO]) donors. CFC-derived colonies from marrow (30 per animal) were picked from methylcellulose. Samples were analyzed by PCR with primers specific for the viral TPO gene and with actin primers to ascertain the presence of material.

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