Truncated and mismatched peptide sequences of TM11, and their affinities for human P-selection as determined by HL60 adhesion assay and competition ELISA
| Peptides . | Name . | HL60* EC50(μM) . | ELISA* IC50 (μM) . |
|---|---|---|---|
| CDVEWVDVSSLEWDLPC | TM11 | 2 | 2 |
| CDVEWVDVS-ahx†-C | A15 | 2 | 4 |
| DVEWVDVS-ahx-S | A16 | 24 | 16 |
| CDVEWVDVSC | A17 | 9 | 5 |
| CDVEWVDVS | A18 | 4 | 7 |
| DVEWVDVS | A19 | 11 | 7 |
| AVEWVDVS | A20 | 12 | 12 |
| DAEWVDVS | A21 | 19 | 6 |
| DVAWVDVS | A22 | 41 | 46 |
| DVEAVDVS | A23 | > 1000 | > 1000 |
| DVEWADVS | A24 | > 1000 | > 1000 |
| DVEWVAVS | A25 | > 1000 | > 1000 |
| DVEWVDAS | A26 | > 1000 | > 1000 |
| DVEWVDVA | A27 | 7 | 6 |
| VEWVDV | A28 | 13 | 10 |
| EWVDV | A29 | 12 | 8 |
| DWVDV | A129 | 23 | 16 |
| KWVDV | A130 | 18 | 28 |
| AWVDV | A131 | 28 | 27 |
| EFVDV | A132 | 654 | 265 |
| EWCDV | A133 | 52 | 115 |
| EWLDV | A134 | 174 | 686 |
| EWVEV | A135 | > 1000 | > 1000 |
| EWVKV | A136 | > 1000 | > 1000 |
| EWVDL | A137 | 59 | 70 |
| WVDV | A138 | > 1000 | > 1000 |
| VDVWE | A139 | > 1000 | > 1000 |
| Peptides . | Name . | HL60* EC50(μM) . | ELISA* IC50 (μM) . |
|---|---|---|---|
| CDVEWVDVSSLEWDLPC | TM11 | 2 | 2 |
| CDVEWVDVS-ahx†-C | A15 | 2 | 4 |
| DVEWVDVS-ahx-S | A16 | 24 | 16 |
| CDVEWVDVSC | A17 | 9 | 5 |
| CDVEWVDVS | A18 | 4 | 7 |
| DVEWVDVS | A19 | 11 | 7 |
| AVEWVDVS | A20 | 12 | 12 |
| DAEWVDVS | A21 | 19 | 6 |
| DVAWVDVS | A22 | 41 | 46 |
| DVEAVDVS | A23 | > 1000 | > 1000 |
| DVEWADVS | A24 | > 1000 | > 1000 |
| DVEWVAVS | A25 | > 1000 | > 1000 |
| DVEWVDAS | A26 | > 1000 | > 1000 |
| DVEWVDVA | A27 | 7 | 6 |
| VEWVDV | A28 | 13 | 10 |
| EWVDV | A29 | 12 | 8 |
| DWVDV | A129 | 23 | 16 |
| KWVDV | A130 | 18 | 28 |
| AWVDV | A131 | 28 | 27 |
| EFVDV | A132 | 654 | 265 |
| EWCDV | A133 | 52 | 115 |
| EWLDV | A134 | 174 | 686 |
| EWVEV | A135 | > 1000 | > 1000 |
| EWVKV | A136 | > 1000 | > 1000 |
| EWVDL | A137 | 59 | 70 |
| WVDV | A138 | > 1000 | > 1000 |
| VDVWE | A139 | > 1000 | > 1000 |
Bold letters indicate the amino acids that differ from the original TM11 peptide sequence.
Results are the average of at least 3 experiments at 10 different concentrations of peptide as determined in the HL60 adhesion assay and TM11-PO competition ELISA (“Materials and methods”)
Ahx (6-aminohexanoic acid) is a C6 spacer.