Most increased transcripts in activated human and mouse MCs
| Name of transcript (protein) . | GenBank accession no.* . | Experiment 1†,‡ . | Experiment 2 . | Experiment 3 . | Fold increase1-153 . | |||
|---|---|---|---|---|---|---|---|---|
| Rest . | Act . | Rest . | Act . | Rest . | Act . | |||
| Human MCs | ||||||||
| Class I MHC-restricted T-cell-associated molecule | AF001622 | <0.11-155 | 4.1 | <0.1 | 5.0 | ND | ND | 676.0 |
| CCL1, I-309 | M57506 | 0.1 | 36.7 | 0.1 | 66.9 | ND | ND | 446.0 |
| GM-CSF | M13207 | 0.1 | 2.5 | 0.4 | 52.6 | ND | ND | 108.6 |
| CCL4, MIP-1β | J04130 | 1.6 | 22.5 | 1.5 | 69.1 | ND | ND | 30.1 |
| CD137, 4-1BB | U03397 | <0.1 | 2.6 | 0.2 | 4.9 | ND | ND | 24.2 |
| Gem GTPase | U10550 | 0.4 | 2.3 | 0.4 | 12.4 | ND | ND | 20.2 |
| CXCL8, IL-8 | M28130 | 4.2 | 20.5 | 2.2 | 78.5 | ND | ND | 15.4 |
| CCL3, MIP-1α | D90144 | 3.0 | 7.8 | 2.0 | 65.5 | ND | ND | 14.5 |
| Putative cyclin G1-interacting protein | U61836 | 0.6 | 7.5 | 1.3 | 19.5 | ND | ND | 13.8 |
| IL-3 | M20137 | 0.8 | 2.6 | 0.3 | 12.9 | ND | ND | 13.5 |
| P1 cre recombinase (3′)1-154 | — | 59.9 | 58.6 | 49.0 | 55.4 | ND | ND | |
| P1 cre recombinase (5′) | — | 42.3 | 39.0 | 30.1 | 40.4 | ND | ND | |
| Mean, 6 AD levels of housekeeping genes | 12710 | 15606 | 17850 | 16398 | ND | ND | ||
| Mouse MCs | ||||||||
| CCL1, I-309 | M23501 | <0.11-155 | 17.5 | <0.1 | 4.2 | 0.2 | 25.1 | 208.2 |
| CD137, 4-1BB | AA798611 | 0.2 | 26.4 | 0.5 | 36.4 | 1.0 | 67.1 | 73.9 |
| CCL7, MCP-3 | X70058 | 0.1 | 21.7 | 0.3 | 14.4 | 0.4 | 15.1 | 60.5 |
| Myeloid differentiation primary response gene 118 | X54149 | 0.9 | 18.2 | <0.1 | 2.4 | 0.3 | 5.6 | 21.4 |
| CCL3, MIP-1α | J04491 | 1.2 | 72.9 | 2.2 | 7.2 | 4.0 | 17.5 | 13.2 |
| CCL4, MIP-1β | X62502 | <0.1 | 23.1 | 1.0 | 6.9 | 3.2 | 12.9 | 10.2 |
| Homeodomain interacting protein kinase 2 | AF077659 | 1.4 | 7.9 | 0.5 | 4.9 | <0.1 | 4.9 | 8.9 |
| Asparagine synthetase | U38940 | 0.8 | 6.1 | 4.2 | 38.0 | 6.2 | 48.3 | 8.3 |
| UI-M-BH2.2-aoo-b-05-0-UI.s1 | AW122030 | 1.3 | 7.4 | 3.1 | 26.2 | 4.8 | 34.4 | 7.4 |
| Secreted phosphoprotein 1 | X13986 | 1.1 | 14.6 | 1.1 | 5.6 | 2.4 | 11.2 | 6.9 |
| P1 cre recombinase (3′)1-154 | — | 112.7 | 83.3 | 133.5 | 152.2 | 112.8 | 91.2 | 58.6 |
| P1 cre recombinase (5′) | — | 72.2 | 56.5 | 75.8 | 83.7 | 66.4 | 48.7 | 39.0 |
| Mean, 6 AD levels of housekeeping genes | 13754 | 18553 | 11767 | 11733 | 9223 | 7819 | ||
| Name of transcript (protein) . | GenBank accession no.* . | Experiment 1†,‡ . | Experiment 2 . | Experiment 3 . | Fold increase1-153 . | |||
|---|---|---|---|---|---|---|---|---|
| Rest . | Act . | Rest . | Act . | Rest . | Act . | |||
| Human MCs | ||||||||
| Class I MHC-restricted T-cell-associated molecule | AF001622 | <0.11-155 | 4.1 | <0.1 | 5.0 | ND | ND | 676.0 |
| CCL1, I-309 | M57506 | 0.1 | 36.7 | 0.1 | 66.9 | ND | ND | 446.0 |
| GM-CSF | M13207 | 0.1 | 2.5 | 0.4 | 52.6 | ND | ND | 108.6 |
| CCL4, MIP-1β | J04130 | 1.6 | 22.5 | 1.5 | 69.1 | ND | ND | 30.1 |
| CD137, 4-1BB | U03397 | <0.1 | 2.6 | 0.2 | 4.9 | ND | ND | 24.2 |
| Gem GTPase | U10550 | 0.4 | 2.3 | 0.4 | 12.4 | ND | ND | 20.2 |
| CXCL8, IL-8 | M28130 | 4.2 | 20.5 | 2.2 | 78.5 | ND | ND | 15.4 |
| CCL3, MIP-1α | D90144 | 3.0 | 7.8 | 2.0 | 65.5 | ND | ND | 14.5 |
| Putative cyclin G1-interacting protein | U61836 | 0.6 | 7.5 | 1.3 | 19.5 | ND | ND | 13.8 |
| IL-3 | M20137 | 0.8 | 2.6 | 0.3 | 12.9 | ND | ND | 13.5 |
| P1 cre recombinase (3′)1-154 | — | 59.9 | 58.6 | 49.0 | 55.4 | ND | ND | |
| P1 cre recombinase (5′) | — | 42.3 | 39.0 | 30.1 | 40.4 | ND | ND | |
| Mean, 6 AD levels of housekeeping genes | 12710 | 15606 | 17850 | 16398 | ND | ND | ||
| Mouse MCs | ||||||||
| CCL1, I-309 | M23501 | <0.11-155 | 17.5 | <0.1 | 4.2 | 0.2 | 25.1 | 208.2 |
| CD137, 4-1BB | AA798611 | 0.2 | 26.4 | 0.5 | 36.4 | 1.0 | 67.1 | 73.9 |
| CCL7, MCP-3 | X70058 | 0.1 | 21.7 | 0.3 | 14.4 | 0.4 | 15.1 | 60.5 |
| Myeloid differentiation primary response gene 118 | X54149 | 0.9 | 18.2 | <0.1 | 2.4 | 0.3 | 5.6 | 21.4 |
| CCL3, MIP-1α | J04491 | 1.2 | 72.9 | 2.2 | 7.2 | 4.0 | 17.5 | 13.2 |
| CCL4, MIP-1β | X62502 | <0.1 | 23.1 | 1.0 | 6.9 | 3.2 | 12.9 | 10.2 |
| Homeodomain interacting protein kinase 2 | AF077659 | 1.4 | 7.9 | 0.5 | 4.9 | <0.1 | 4.9 | 8.9 |
| Asparagine synthetase | U38940 | 0.8 | 6.1 | 4.2 | 38.0 | 6.2 | 48.3 | 8.3 |
| UI-M-BH2.2-aoo-b-05-0-UI.s1 | AW122030 | 1.3 | 7.4 | 3.1 | 26.2 | 4.8 | 34.4 | 7.4 |
| Secreted phosphoprotein 1 | X13986 | 1.1 | 14.6 | 1.1 | 5.6 | 2.4 | 11.2 | 6.9 |
| P1 cre recombinase (3′)1-154 | — | 112.7 | 83.3 | 133.5 | 152.2 | 112.8 | 91.2 | 58.6 |
| P1 cre recombinase (5′) | — | 72.2 | 56.5 | 75.8 | 83.7 | 66.4 | 48.7 | 39.0 |
| Mean, 6 AD levels of housekeeping genes | 13754 | 18553 | 11767 | 11733 | 9223 | 7819 | ||
Unreviewed additional data are shown athttp://www.nch.go.jp/imal/English_index.htm.
Rest indicates resting; Act, active; MHC, major histocompatibility complex; GTPase, guanosine triphosphatase; AD, average difference; and ND, not done.
The GenBank is accessible at http://www.ncbi.nlm.nih.gov.
Three different batches of human cord blood-derived cultured MCs at week 14 (experiment 1) and week 11 (experiment 2) were primed with SCF, IL-3, IL-6, and IL-4 (see “Materials and methods”). Sensitized MCs were then challenged with either anti-IgE (activated) or control medium (resting) for 6 hours.
Balb/c mouse bone marrow-derived cultured MCs at week 4 (experiment 1) with IL-3 were further cultured with IL-3 plus SCF for 1 week (experiment 2). NOA mouse bone marrow-derived cultured MCs at week 4 with IL-3 were also cultured with SCF and IL-3 (see “Materials and methods”). Sensitized MCs were then challenged with either DNP-BSA antigen (activated) or control medium (resting) for 6 hours.
Fold increase was obtained by calculating the AD levels of activated MCs vs those of resting MCs.
The percentages of the specific average AD level versus the mean AD level of 6 probe sets for housekeeping genes are shown. Only transcripts that expressed more than 2% of housekeeping gene levels and that were judged to be present in activated cells are shown in this table.
External standards such as P1 cre recombinase protein were added to cRNA of the target sample just prior to the hybridization (see “Materials and methods”) to evaluate the difference between samples. These standards were found at similar levels (an increase of less than 2-fold) in these MC samples after compensation for the housekeeping genes.