mRNA was isolated from sorted populations of bone marrow macrophages, neutrophils, and lymphocytes and converted into cDNA. Semiquantitative RT-PCR was performed with primers specific for the A2BAR and β-actin using three 5-fold serial dilutions of cDNA. The data are expressed as the mean plus or minus SEM of the ratio of A2BAR mRNA/β-actin mRNA for three determinations.