Inhibition of clonogenic growth of primary CML cells by SMA-ZnPP
| . | Control medium . | SMA-ZnPP . | ||||
|---|---|---|---|---|---|---|
| CFU-GM . | BFU-E . | CFU-GEMM . | CFU-GM . | BFU-E . | CFU-GEMM . | |
| CML (MNC) no. 1 | 29 | 133 | 3 | 0 | 0 | 0 |
| CML (MNC) no. 2 | 18 | 160 | 0 | 0 | 0 | 0 |
| CML (MNC) no. 3 | 17 | 59 | 0 | 0 | 0 | 0 |
| Healthy donor (MNC) no. 1 | 6 | 27 | 1 | 1 | 20 | 0 |
| Healthy donor (MNC) no. 2 | 9 | 36 | 2 | 2 | 15 | 1 |
| Healthy donor (MNC) no. 3 | 8 | 37 | 0 | 5 | 35 | 1 |
| Healthy donor (CD34+ cells) | 43 | 110 | 2 | 31 | 106 | 1 |
| CML (CD34+ cells) no. 1 | 12 | 107 | 3 | 0 | 0 | 0 |
| CML (CD34+ cells) no. 2 | 21 | 210 | 0 | 0 | 9 | 0 |
| CML (CD34+ cells) no. 3 | 18 | 22 | 0 | 2 | 5 | 0 |
| . | Control medium . | SMA-ZnPP . | ||||
|---|---|---|---|---|---|---|
| CFU-GM . | BFU-E . | CFU-GEMM . | CFU-GM . | BFU-E . | CFU-GEMM . | |
| CML (MNC) no. 1 | 29 | 133 | 3 | 0 | 0 | 0 |
| CML (MNC) no. 2 | 18 | 160 | 0 | 0 | 0 | 0 |
| CML (MNC) no. 3 | 17 | 59 | 0 | 0 | 0 | 0 |
| Healthy donor (MNC) no. 1 | 6 | 27 | 1 | 1 | 20 | 0 |
| Healthy donor (MNC) no. 2 | 9 | 36 | 2 | 2 | 15 | 1 |
| Healthy donor (MNC) no. 3 | 8 | 37 | 0 | 5 | 35 | 1 |
| Healthy donor (CD34+ cells) | 43 | 110 | 2 | 31 | 106 | 1 |
| CML (CD34+ cells) no. 1 | 12 | 107 | 3 | 0 | 0 | 0 |
| CML (CD34+ cells) no. 2 | 21 | 210 | 0 | 0 | 9 | 0 |
| CML (CD34+ cells) no. 3 | 18 | 22 | 0 | 2 | 5 | 0 |
Primary mononuclear cells (MNC) were isolated from the peripheral blood of untreated CML patients or from healthy controls by Ficoll density centrifugation and were then cultured in control medium or SMA-ZnPP (10 μM) for 24 hours. Thereafter, cells were cultured in methylcellulose in the presence of cytokines as described in the text for 14 days. Then, cultures were examined for the number of colonies by an inverted microscope. Results show the numbers of colonies per 105 cells (MNC seeded on day 0) or colonies per 5 x 103 CD34+ cells and represent the mean of duplicates in each donor.