Response of leukemic cell lines to Hsp32/HO-1 inhibitors
| Cell line . | PEG-ZnPP (μM) . | SMA-ZnPP (μM) . | ZnDPPIX (μM) . | |||
|---|---|---|---|---|---|---|
| IC50 . | Range . | IC50 . | Range . | IC50 . | Range . | |
| K562 | 3 | 2–4 | 6 | 4–8 | 8 | 7–10 |
| KU812 | 5 | 2–7 | 7 | 2–12 | 5 | 4–6 |
| Imatinib-resistant K562 | 4 | 3–6 | 7 | 4–8 | 24 | 20–30 |
| BaF3p210wt | n.t | n.t | 16 | 5–28 | n.t | n.t |
| BaF3p210T315I | n.t | n.t | 8 | 5–10 | n.t | n.t |
| BaF3p210E255K | n.t | n.t | 10 | 5–13 | n.t | n.t |
| BaF3p210Y253F | n.t | n.t | 15 | 10–25 | n.t | n.t |
| BaF3p210M351T | n.t | n.t | 13 | 5–18 | n.t | n.t |
| BaF3p210H396P | n.t | n.t | 6 | 5–8 | n.t | n.t |
| Cell line . | PEG-ZnPP (μM) . | SMA-ZnPP (μM) . | ZnDPPIX (μM) . | |||
|---|---|---|---|---|---|---|
| IC50 . | Range . | IC50 . | Range . | IC50 . | Range . | |
| K562 | 3 | 2–4 | 6 | 4–8 | 8 | 7–10 |
| KU812 | 5 | 2–7 | 7 | 2–12 | 5 | 4–6 |
| Imatinib-resistant K562 | 4 | 3–6 | 7 | 4–8 | 24 | 20–30 |
| BaF3p210wt | n.t | n.t | 16 | 5–28 | n.t | n.t |
| BaF3p210T315I | n.t | n.t | 8 | 5–10 | n.t | n.t |
| BaF3p210E255K | n.t | n.t | 10 | 5–13 | n.t | n.t |
| BaF3p210Y253F | n.t | n.t | 15 | 10–25 | n.t | n.t |
| BaF3p210M351T | n.t | n.t | 13 | 5–18 | n.t | n.t |
| BaF3p210H396P | n.t | n.t | 6 | 5–8 | n.t | n.t |
Cell lines were cultured in control medium or in various concentrations of Hsp32/HO-1 inhibitors for 48 hours. Thereafter, proliferation of cells was measured by 3H-thymidine incorporation assay. IC50 values (μM) represent the mean from at least 3 independent experiments. The ranges of IC50 values (μM) are also provided.
n.t. indicates not tested.