Partial cell-cycle arrest of adherent MMSET knockdown cells
| Condition . | G0/G1, % . | S, % . | G2/M, % . |
|---|---|---|---|
| Control suspension | 47.74 ± 0.95 | 43.99 ± 1.10 | 8.28 ± 2.05 |
| Knockdown suspension | 46.44 ± 5.62 | 41.60 ± 3.81 | 11.97 ± 1.81 |
| Control adherent | 32.69 ± 3.04 | 57.17 ± 0.05 | 10.15 ± 2.99 |
| Knockdown adherent | 43.52 ± 8.46 | 40.88 ± 4.44* | 15.60 ± 3.99 |
| Condition . | G0/G1, % . | S, % . | G2/M, % . |
|---|---|---|---|
| Control suspension | 47.74 ± 0.95 | 43.99 ± 1.10 | 8.28 ± 2.05 |
| Knockdown suspension | 46.44 ± 5.62 | 41.60 ± 3.81 | 11.97 ± 1.81 |
| Control adherent | 32.69 ± 3.04 | 57.17 ± 0.05 | 10.15 ± 2.99 |
| Knockdown adherent | 43.52 ± 8.46 | 40.88 ± 4.44* | 15.60 ± 3.99 |
Cell cycle profiling by flow cytometric analysis of DNA content was performed on KMS-11 cells transduced with empty vector lentivirus or MMSET shRNA lentivirus growing in suspension or adherent. Percentages of G0/G1, S, and G2/M phase cells were calculated with Modfit LT software. Results are the average of 2 experiments derived from independent lentiviral infections. Values are mean (± SD).
P = .03 versus control adherent.