Oligonucleotides used for cloning and site-directed mutagenesis to generate retroviral expression vectors
| Name . | Sequence . |
|---|---|
| mRsk1Kozak-T | ATA TAT CTC GAG GCC GCC ACC ATG CCG CTC GCC CAG CTC AAG |
| mRsk1-B | ATA TAT CTC GAG TCA CAG GGT GGT GGA TGG CAG CTT CC |
| mRsk1 K94A-T | GGG CAC TTG TAT GCC ATG GCA GTA TTA AAG AAG GCC ACG |
| mRsk1 K94A-B | CGT GGC CTT CTT TAA TAC TGC CAT GGC ATA CAA GTG CCC |
| mRsk1 K447A-T | ATG GAG TAT GCT GTC GCG GTC ATC GAC AAG AGC |
| mRsk2 Kozak: | GGA ATT AGA TCT CTC GAG GCC GCC ACC ATG CCG CTG GCG CAG CTG |
| mRsk2 K100A-T: | AGA CAG CTT TAT GCC ATG GCA GTA TTA AAG AAG GCC ACG CTG |
| mRsk2 K100A-B: | CAG CGT GGC CTT CTT TAA TAC TGC CAT GGC ATA AAG CTG TCT |
| mRsk2 K451A-T: | AAC ATG GAG TTT GCC GTG GCG ATT ATT GAT AAA AGC AAG AG |
| mRsk2 K451A-B: | CTC TTG CTT TTA TCA ATA ATC GCC ACG GCA AAC TCC ATG TT |
| mRsk1 K447A-B | GCT CTT GTC GAT GAC CGC GAC AGC ATA CTC CAT |
| mRsk3 Kozak-T | ATA TAT GTC GAC GCC GCC ACC ATG GAG CTG AGC ATG AAG AAG TTC |
| mRsk3-B | ATA TAT GTC GAC CTA CAA CCT GGT AGA CGT GAG TCT C |
| mRsk3 | K91A-T GGT CAG CTC TAC GCC ATG GCG GTC CTG AAG AAA GCC ACC |
| mRsk3 | K91A-B GGT GGC TTT CTT CAG GAC CGC CAT GGC GTA GAG CTG ACC |
| mRsk3 | K444A-T GAT GCT GAG TAT GCT GTG GCG ATC ATC GAT AAG AGC AAA |
| mRsk3 | K444A-B TTT GCT CTT ATC GAT GAT CGC CAC AGC ATA CTC AGC GTC |
| mRsk1 121 | CAC CCG TTC GTG GTG AAG |
| mRsk1 271 | CCC CAG TTT CTG AGC ACG |
| mRsk1 421 | TGT AAG CGT TGT GTC CAC |
| mRsk1 571 | GCA CCT GAG GTC CTG AAG |
| mRsk2 121 | ATC TTG GTA GAA GTC AAT C |
| mRsk2 271 | AAA GAT CGT AAA GAA ACA |
| mRsk2 421 | GGA TAT GAA GTA AAA GAG |
| mRsk2 571 | GAA AAT GGT CTT CTC ATG |
| mRsk3 121 | ATT GTC AAG CTG CAT TAT G |
| mRsk3 271 | ATC CTC AAA GCC AAG CTG |
| mRsk3 421 | ATC GGG GTG GGC TCC TAC |
| mRsk3 571 | CCC TGC TAT ACT GCA AAC |
| pRV 5′ Seq: | TTA TCC AGC CCT CAC TCC TTC |
| Name . | Sequence . |
|---|---|
| mRsk1Kozak-T | ATA TAT CTC GAG GCC GCC ACC ATG CCG CTC GCC CAG CTC AAG |
| mRsk1-B | ATA TAT CTC GAG TCA CAG GGT GGT GGA TGG CAG CTT CC |
| mRsk1 K94A-T | GGG CAC TTG TAT GCC ATG GCA GTA TTA AAG AAG GCC ACG |
| mRsk1 K94A-B | CGT GGC CTT CTT TAA TAC TGC CAT GGC ATA CAA GTG CCC |
| mRsk1 K447A-T | ATG GAG TAT GCT GTC GCG GTC ATC GAC AAG AGC |
| mRsk2 Kozak: | GGA ATT AGA TCT CTC GAG GCC GCC ACC ATG CCG CTG GCG CAG CTG |
| mRsk2 K100A-T: | AGA CAG CTT TAT GCC ATG GCA GTA TTA AAG AAG GCC ACG CTG |
| mRsk2 K100A-B: | CAG CGT GGC CTT CTT TAA TAC TGC CAT GGC ATA AAG CTG TCT |
| mRsk2 K451A-T: | AAC ATG GAG TTT GCC GTG GCG ATT ATT GAT AAA AGC AAG AG |
| mRsk2 K451A-B: | CTC TTG CTT TTA TCA ATA ATC GCC ACG GCA AAC TCC ATG TT |
| mRsk1 K447A-B | GCT CTT GTC GAT GAC CGC GAC AGC ATA CTC CAT |
| mRsk3 Kozak-T | ATA TAT GTC GAC GCC GCC ACC ATG GAG CTG AGC ATG AAG AAG TTC |
| mRsk3-B | ATA TAT GTC GAC CTA CAA CCT GGT AGA CGT GAG TCT C |
| mRsk3 | K91A-T GGT CAG CTC TAC GCC ATG GCG GTC CTG AAG AAA GCC ACC |
| mRsk3 | K91A-B GGT GGC TTT CTT CAG GAC CGC CAT GGC GTA GAG CTG ACC |
| mRsk3 | K444A-T GAT GCT GAG TAT GCT GTG GCG ATC ATC GAT AAG AGC AAA |
| mRsk3 | K444A-B TTT GCT CTT ATC GAT GAT CGC CAC AGC ATA CTC AGC GTC |
| mRsk1 121 | CAC CCG TTC GTG GTG AAG |
| mRsk1 271 | CCC CAG TTT CTG AGC ACG |
| mRsk1 421 | TGT AAG CGT TGT GTC CAC |
| mRsk1 571 | GCA CCT GAG GTC CTG AAG |
| mRsk2 121 | ATC TTG GTA GAA GTC AAT C |
| mRsk2 271 | AAA GAT CGT AAA GAA ACA |
| mRsk2 421 | GGA TAT GAA GTA AAA GAG |
| mRsk2 571 | GAA AAT GGT CTT CTC ATG |
| mRsk3 121 | ATT GTC AAG CTG CAT TAT G |
| mRsk3 271 | ATC CTC AAA GCC AAG CTG |
| mRsk3 421 | ATC GGG GTG GGC TCC TAC |
| mRsk3 571 | CCC TGC TAT ACT GCA AAC |
| pRV 5′ Seq: | TTA TCC AGC CCT CAC TCC TTC |
Underlined nucleotides are mutations. Sequencing primers are also listed.