Table 1 Properties of anti-C2 MAbs . MAb... | American Society of Hematology

Table 1

Properties of anti-C2 MAbs

	MAb	Spleen, no.*	Isotype	Group	Inhibitor titer, BU/mg	Type	IC₅₀ VWF binding, μg/mL	IC₅₀ phospholipid binding, μg/mL
1	3E6	1	IgG2aκ	A	41	I	0.6	0.4
2	I54	7	IgG2bκ	A	1 300	I	0.02	0.02
3	ESH4	†	IgG2a	A	39	II	1	0.5
4	F85	5	IgG1κ	AB	6	II	1.9	1.8
5	F100	5	IgG1κ	AB	5	II	2	1.2
6	F137	5	IgG2aκ	AB	6	IND	1.5	1.1
7	I89	7	IgG2aκ	AB	1 900	I	0.02	0.03
8	I117	7	IgG2aκ	AB	1 800	I	0.02	0.03
9	I109	7	IgG1κ	AB	1 500	I	0.04	0.07
10	BO2C11	†	IgG4	AB	20 000	I	0.007	0.01
11	1B5	1	IgG2aκ	B	930	II	0.05	0.03
12	3C6	1	IgG2aκ	B	71	IND	3.4	0.3
13	3D12	1	IgG2bκ	B	2 600	I	0.04	0.05
14	D102	4	IgG2aκ	B	3 800	I	0.03	0.02
15	3G6	1	IgG2bκ	BC	25 000	II	8.1	4.8
16	2–77	2	IgG2aκ	BC	25 000	II	>10	>10
17	B45	3	IgG2bκ	BC	21 000	II	>10	>10
18	B9	3	IgG2aκ	BC	31 000	II	>10	0.4
19	B11	3	IgG1κ	BC	3 300	II	>10	>10
20	B75	3	IgG2aκ	BC	IND	II	2.4	2.4
21	D105	4	IgG1κ	BC	0.8	II	>10	>10
22	F77	5	IgG2bκ	BC	26 000	II	>10	>10
23	F178	5	IgG1κ	BC	18 000	II	>10	>10
24	F67	5	IgG2bκ	BC	21 000	II	>10	>10
25	G99	6	IgG2aκ	BC	15 000	II	>10	>10
26	G86	6	IgG2aκ	BC	4 300	II	>10	4.1
27	I14	7	IgG2aκ	BC	44 000	II	>10	>10
28	I55	7	IgG1κ	BC	10 000	II	0.02	>10
29	2–117	2	IgG2aκ	C	>0.4	II	>10	>10
30	ESH8	†	IgG2a	C	10 000	II	>10	>10

	MAb	Spleen, no.*	Isotype	Group	Inhibitor titer, BU/mg	Type	IC₅₀ VWF binding, μg/mL	IC₅₀ phospholipid binding, μg/mL
1	3E6	1	IgG2aκ	A	41	I	0.6	0.4
2	I54	7	IgG2bκ	A	1 300	I	0.02	0.02
3	ESH4	†	IgG2a	A	39	II	1	0.5
4	F85	5	IgG1κ	AB	6	II	1.9	1.8
5	F100	5	IgG1κ	AB	5	II	2	1.2
6	F137	5	IgG2aκ	AB	6	IND	1.5	1.1
7	I89	7	IgG2aκ	AB	1 900	I	0.02	0.03
8	I117	7	IgG2aκ	AB	1 800	I	0.02	0.03
9	I109	7	IgG1κ	AB	1 500	I	0.04	0.07
10	BO2C11	†	IgG4	AB	20 000	I	0.007	0.01
11	1B5	1	IgG2aκ	B	930	II	0.05	0.03
12	3C6	1	IgG2aκ	B	71	IND	3.4	0.3
13	3D12	1	IgG2bκ	B	2 600	I	0.04	0.05
14	D102	4	IgG2aκ	B	3 800	I	0.03	0.02
15	3G6	1	IgG2bκ	BC	25 000	II	8.1	4.8
16	2–77	2	IgG2aκ	BC	25 000	II	>10	>10
17	B45	3	IgG2bκ	BC	21 000	II	>10	>10
18	B9	3	IgG2aκ	BC	31 000	II	>10	0.4
19	B11	3	IgG1κ	BC	3 300	II	>10	>10
20	B75	3	IgG2aκ	BC	IND	II	2.4	2.4
21	D105	4	IgG1κ	BC	0.8	II	>10	>10
22	F77	5	IgG2bκ	BC	26 000	II	>10	>10
23	F178	5	IgG1κ	BC	18 000	II	>10	>10
24	F67	5	IgG2bκ	BC	21 000	II	>10	>10
25	G99	6	IgG2aκ	BC	15 000	II	>10	>10
26	G86	6	IgG2aκ	BC	4 300	II	>10	4.1
27	I14	7	IgG2aκ	BC	44 000	II	>10	>10
28	I55	7	IgG1κ	BC	10 000	II	0.02	>10
29	2–117	2	IgG2aκ	C	>0.4	II	>10	>10
30	ESH8	†	IgG2a	C	10 000	II	>10	>10

IND indicates indeterminate.

Spleen numbers refer to the 7 mice that were immunized with human fVIII as described in “Anti-fVIII MAbs from anti-fVIII hybridomas.”

ESH4 and ESH8 are commercial murine MAbs; BO2C11 is a human MAb.