Composition of EAP
| Protein . | Accession no. . | Molecular weight, kDa . | Peptides, no.* . | Remarks . |
|---|---|---|---|---|
| BCoR | NP_060215 | 188 | 13 | BCL-6–interacting corepressor |
| DOT1L | NP_115871 | 165 | 11 | Histone H3K79 methyltransferase |
| LAF4 | NP_002276 | 133 | 26 | MLL fusion partner, AF4 and AF5q31 homolog |
| AF4 | NP_005926 | 131 | 110 | MLL fusion partner, AF5q31 homolog |
| AF5q31 | NP_055238 | 127 | 741 | MLL fusion partner, AF4 homolog |
| CYCT2 | NP_490595 | 81 | 52 | Cyclin T2, subunit of pTEF-b |
| HSP70 | NP_940833 | 70 | 169 | Heat-shock protein |
| HSP70L | NP_005336 | 70 | 6 | Heat-shock protein–like |
| ENL | NP_005925 | 62 | 167 | MLL fusion partner, tagged for purification |
| p52 RO | NP_003132 | 54 | 47 | Ubiquitin ligase, E3 enzyme |
| TUB6 | NP_997195 | 50 | 3 | Tubulin variant |
| PIP5K2C | NP_079055 | 47 | 2 | Phosphatidylinositol-4-phosphate-5-kinase, type II, γ |
| CBX8 | NP_065700 | 43 | 3 | Chromobox 8 protein |
| CDK9 | NP_001252 | 43 | 55 | Subunit of pTEF-b, RNAPol II CTD kinase |
| RING1 | NP_002922 | 42 | 10 | Member of polycomb repressive complex I |
| Protein . | Accession no. . | Molecular weight, kDa . | Peptides, no.* . | Remarks . |
|---|---|---|---|---|
| BCoR | NP_060215 | 188 | 13 | BCL-6–interacting corepressor |
| DOT1L | NP_115871 | 165 | 11 | Histone H3K79 methyltransferase |
| LAF4 | NP_002276 | 133 | 26 | MLL fusion partner, AF4 and AF5q31 homolog |
| AF4 | NP_005926 | 131 | 110 | MLL fusion partner, AF5q31 homolog |
| AF5q31 | NP_055238 | 127 | 741 | MLL fusion partner, AF4 homolog |
| CYCT2 | NP_490595 | 81 | 52 | Cyclin T2, subunit of pTEF-b |
| HSP70 | NP_940833 | 70 | 169 | Heat-shock protein |
| HSP70L | NP_005336 | 70 | 6 | Heat-shock protein–like |
| ENL | NP_005925 | 62 | 167 | MLL fusion partner, tagged for purification |
| p52 RO | NP_003132 | 54 | 47 | Ubiquitin ligase, E3 enzyme |
| TUB6 | NP_997195 | 50 | 3 | Tubulin variant |
| PIP5K2C | NP_079055 | 47 | 2 | Phosphatidylinositol-4-phosphate-5-kinase, type II, γ |
| CBX8 | NP_065700 | 43 | 3 | Chromobox 8 protein |
| CDK9 | NP_001252 | 43 | 55 | Subunit of pTEF-b, RNAPol II CTD kinase |
| RING1 | NP_002922 | 42 | 10 | Member of polycomb repressive complex I |
The table lists proteins that could be uniquely identified by more than 1 peptide and at least in 2 independent EAP samples. In addition, peptides specific for the MLL fusion partners ELL (accession no. NP_006523), AF9 (accession no. NP_004520), and AF10 (accession no. NP_004632) were detected in single experiments. The given molecular weight corresponds to the theoretical calculated value of the prototypical protein as deposited in the database.
All gene-specific peptides that could be unambigously identified by mass-spectrometry across all replicates.