Syk interactome in AML cells
| Ligand . | IPI no. . | Function . | Found in B cells . |
|---|---|---|---|
| STAT5A | 00030783 | Transcription factor | No |
| STAT3 | 00784414 | Transcription factor | No |
| Cbl | 00884054 | E3 ubiquitin ligase | Yes45 |
| Vav | 00011696 | Guanosine exchange factor | Yes46 |
| PI3K p85 | 00807573 | Regulatory subunit of PI3-kinase | Yes47 |
| PIK3AP1 | 00783519 | Adaptor for PI3-kinase | No |
| 14-3-3-γ | 00220642 | Adaptor protein | Yes26 |
| 14-3-3-ζ/-δ | 00982101 | Adaptor protein | Yes26 |
| Ibtk | 00792333 | Inhibitor of Btk | No |
| FcγRI | 00023502 | High affinity Fc receptor | No |
| Integrin β2 | 00291792 | Subunit of Mac-1 | No |
| Integrin, α M | 00645887 | Subunit of Mac-1 | No |
| Tubulin α-4a | 00007750 | Cytoskeleton component | Yes26,27 |
| Tubulin α-1C | 00218343 | Cytoskeleton component | Yes26,27 |
| Tubulin β-2C | 00007752 | Cytoskeleton component | Yes26,27 |
| Tubulin β chain | 01019113 | Cytoskeleton component | Yes26,27 |
| Dynein 1 intermediate chain-2 | 00744015 | Motor protein | Yes26,27 |
| Dynein 1 heavy chain-1 | 00456969 | Motor protein | Yes26,27 |
| Ligand . | IPI no. . | Function . | Found in B cells . |
|---|---|---|---|
| STAT5A | 00030783 | Transcription factor | No |
| STAT3 | 00784414 | Transcription factor | No |
| Cbl | 00884054 | E3 ubiquitin ligase | Yes45 |
| Vav | 00011696 | Guanosine exchange factor | Yes46 |
| PI3K p85 | 00807573 | Regulatory subunit of PI3-kinase | Yes47 |
| PIK3AP1 | 00783519 | Adaptor for PI3-kinase | No |
| 14-3-3-γ | 00220642 | Adaptor protein | Yes26 |
| 14-3-3-ζ/-δ | 00982101 | Adaptor protein | Yes26 |
| Ibtk | 00792333 | Inhibitor of Btk | No |
| FcγRI | 00023502 | High affinity Fc receptor | No |
| Integrin β2 | 00291792 | Subunit of Mac-1 | No |
| Integrin, α M | 00645887 | Subunit of Mac-1 | No |
| Tubulin α-4a | 00007750 | Cytoskeleton component | Yes26,27 |
| Tubulin α-1C | 00218343 | Cytoskeleton component | Yes26,27 |
| Tubulin β-2C | 00007752 | Cytoskeleton component | Yes26,27 |
| Tubulin β chain | 01019113 | Cytoskeleton component | Yes26,27 |
| Dynein 1 intermediate chain-2 | 00744015 | Motor protein | Yes26,27 |
| Dynein 1 heavy chain-1 | 00456969 | Motor protein | Yes26,27 |
Two independent SILAC-based liquid chromatography-tandem mass spectrometry analyses were performed for identification of Syk interactors in untreated KG-1 cells. Mass spectrometric data sets were analyzed by MaxQuant software. The proteins listed showed an at least fivefold enrichment of heavy peptides compared with light ones. The protein functions listed were obtained by a literature search. In addition the international protein index number (IPI no.) is listed for each identified protein. Detailed statistics including total numbers of all identified and quantified peptides are outlined in supplemental Table 4.