Enrichr analysis of scRNAseq data
| Gene fraction . | Enriched terms* . | Adjusted P . | Source† . |
|---|---|---|---|
| A | Processes/pathways | ||
| Top 200 expressed genes (all cells) | Hemoglobin’s chaperone_Homo sapiens_h_ahspPathway | 6.46E−10 | BioCarta 2016 |
| Heme biosynthesis_Homo sapiens_PWY-5920 | 1.82E−03 | HumanCyc 2016 | |
| Heme biosynthesis_Homo sapiens_P02746 | 1.39E−01 | Panther 2016 | |
| Hypoxic and oxygen homeostasis regulation of HIF-1-alpha_Homo sapiens_4c0f3584-6193-11e5-8ac5-06603eb7f303 | 5.54E−02 | NCI-Nature 2016 | |
| Ribosome_Homo sapiens_hsa03010 | 7.22E−41 | KEGG 2016 | |
| HIF-1 signaling pathway_Homo sapiens_hsa04066 | 2.75E−02 | KEGG 2016 | |
| Upregulated in GFP+ | Hemoglobin’s chaperone_Homo sapiens_h_ahspPathway | 8.26E−07 | BioCarta 2016 |
| Heme biosynthesis_Homo sapiens_PWY-5920 | 4.54E−02 | HumanCyc 2016 | |
| Heme biosynthesis_Homo sapiens_P02746 | 7.41E−02 | Panther 2016 | |
| Ubiquitin protein ligase binding (GO:0031625) | 4.20E−02 | GO molecular function 2017 | |
| Oxygen transport (GO:0015671) | 1.29E−04 | GO biological process 2015 | |
| Oxidative stress_Homo sapiens_WP408 | 1.12E−02 | WikiPathways 2016 | |
| Cellular responses to stress_Homo sapiens_R-HSA-2262752 | 2.82E−04 | Reactome 2016 | |
| HIF-1-α transcription factor network_Homo sapiens_20ef2b81-6193-11e5-8ac5-06603eb7f303 | 5.57E−04 | NCI-Nature 2016 | |
| Downregulated in GFP+ | Ribosome_Homo sapiens_hsa03010 | 4.75E−29 | KEGG 2016 |
| tRNA binding (GO:0000049) | 6.01E−16 | GO molecular function 2017b | |
| Peptide chain elongation_Homo sapiens_R-HSA-156902 | 1.52E−31 | Reactome 2016 | |
| Eukaryotic Translation Elongation_Homo sapiens_R-HSA-156842 | 1.71E−31 | Reactome 2016 | |
| Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)_Homo sapiens_R-HSA-975956 | 1.71E−31 | Reactome 2016 | |
| 3′ -UTR-mediated translational regulation_Homo sapiens_R-HSA-157279 | 5.82E−30 | Reactome 2016 | |
| B | Cell type | ||
| Top 200 expressed genes (all cells) | CD71+_EarlyErythroid | 1.90E−23 | Human Gene Atlas |
| Bone marrow (bulk tissue) | 2.98E−25 | ARCHS4 Tissues | |
| Cord blood | 1.60E−23 | ARCHS4 Tissues | |
| Erythroblast | 1.07E−03 | ARCHS4 Tissues | |
| Upregulated in GFP+ | CD71+_EarlyErythroid | 4.25E−49 | Human Gene Atlas |
| Erythroid_cell | 6.60E−30 | Jensen Tissues | |
| Blood | 7.61E−13 | Jensen Tissues | |
| Erythroblast | 1.96E−12 | ARCHS4 Tissues | |
| C | Transcription factor targets | ||
| Upregulated in GFP+ | KLF1 | 5.196E−32 | Enrichr Submissions TF-Gene Coocurrence |
| KLF1_20508144_ChIP-Seq_FETAL-LIVER-ERYTHROID_Mouse | 1.490E−06 | ChEA 2016 | |
| GATA1_CHEA | 4.279E−15 | ENCODE and ChEA | |
| GATA1_erythroblast_hg19 | 7.311E−9 | Consensus TFs from ChIP-X | |
| GATA1_19941826_ChIP-Seq_K562_Human | 4.508E−8 | ChEA 2016 | |
| TAL1_20566737_ChIP-Seq_PRIMARY_FETAL_LIVER_ ERYTHROID_Mouse | 5.805E−12 | ChEA 2016 | |
| TAL1_erythroblast_mm9 | 4.769E−10 | ENCODE TF ChIP-seq 2015 |
| Gene fraction . | Enriched terms* . | Adjusted P . | Source† . |
|---|---|---|---|
| A | Processes/pathways | ||
| Top 200 expressed genes (all cells) | Hemoglobin’s chaperone_Homo sapiens_h_ahspPathway | 6.46E−10 | BioCarta 2016 |
| Heme biosynthesis_Homo sapiens_PWY-5920 | 1.82E−03 | HumanCyc 2016 | |
| Heme biosynthesis_Homo sapiens_P02746 | 1.39E−01 | Panther 2016 | |
| Hypoxic and oxygen homeostasis regulation of HIF-1-alpha_Homo sapiens_4c0f3584-6193-11e5-8ac5-06603eb7f303 | 5.54E−02 | NCI-Nature 2016 | |
| Ribosome_Homo sapiens_hsa03010 | 7.22E−41 | KEGG 2016 | |
| HIF-1 signaling pathway_Homo sapiens_hsa04066 | 2.75E−02 | KEGG 2016 | |
| Upregulated in GFP+ | Hemoglobin’s chaperone_Homo sapiens_h_ahspPathway | 8.26E−07 | BioCarta 2016 |
| Heme biosynthesis_Homo sapiens_PWY-5920 | 4.54E−02 | HumanCyc 2016 | |
| Heme biosynthesis_Homo sapiens_P02746 | 7.41E−02 | Panther 2016 | |
| Ubiquitin protein ligase binding (GO:0031625) | 4.20E−02 | GO molecular function 2017 | |
| Oxygen transport (GO:0015671) | 1.29E−04 | GO biological process 2015 | |
| Oxidative stress_Homo sapiens_WP408 | 1.12E−02 | WikiPathways 2016 | |
| Cellular responses to stress_Homo sapiens_R-HSA-2262752 | 2.82E−04 | Reactome 2016 | |
| HIF-1-α transcription factor network_Homo sapiens_20ef2b81-6193-11e5-8ac5-06603eb7f303 | 5.57E−04 | NCI-Nature 2016 | |
| Downregulated in GFP+ | Ribosome_Homo sapiens_hsa03010 | 4.75E−29 | KEGG 2016 |
| tRNA binding (GO:0000049) | 6.01E−16 | GO molecular function 2017b | |
| Peptide chain elongation_Homo sapiens_R-HSA-156902 | 1.52E−31 | Reactome 2016 | |
| Eukaryotic Translation Elongation_Homo sapiens_R-HSA-156842 | 1.71E−31 | Reactome 2016 | |
| Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)_Homo sapiens_R-HSA-975956 | 1.71E−31 | Reactome 2016 | |
| 3′ -UTR-mediated translational regulation_Homo sapiens_R-HSA-157279 | 5.82E−30 | Reactome 2016 | |
| B | Cell type | ||
| Top 200 expressed genes (all cells) | CD71+_EarlyErythroid | 1.90E−23 | Human Gene Atlas |
| Bone marrow (bulk tissue) | 2.98E−25 | ARCHS4 Tissues | |
| Cord blood | 1.60E−23 | ARCHS4 Tissues | |
| Erythroblast | 1.07E−03 | ARCHS4 Tissues | |
| Upregulated in GFP+ | CD71+_EarlyErythroid | 4.25E−49 | Human Gene Atlas |
| Erythroid_cell | 6.60E−30 | Jensen Tissues | |
| Blood | 7.61E−13 | Jensen Tissues | |
| Erythroblast | 1.96E−12 | ARCHS4 Tissues | |
| C | Transcription factor targets | ||
| Upregulated in GFP+ | KLF1 | 5.196E−32 | Enrichr Submissions TF-Gene Coocurrence |
| KLF1_20508144_ChIP-Seq_FETAL-LIVER-ERYTHROID_Mouse | 1.490E−06 | ChEA 2016 | |
| GATA1_CHEA | 4.279E−15 | ENCODE and ChEA | |
| GATA1_erythroblast_hg19 | 7.311E−9 | Consensus TFs from ChIP-X | |
| GATA1_19941826_ChIP-Seq_K562_Human | 4.508E−8 | ChEA 2016 | |
| TAL1_20566737_ChIP-Seq_PRIMARY_FETAL_LIVER_ ERYTHROID_Mouse | 5.805E−12 | ChEA 2016 | |
| TAL1_erythroblast_mm9 | 4.769E−10 | ENCODE TF ChIP-seq 2015 |
Enriched in different fractions of day-29 β_GFP reporter hiPSC-derived erythroid cells according to Enrichr analysis of scRNAseq data.
Gene set libraries were accessed through the Enrichr platform (http://amp.pharm.mssm.edu/Enrichr/).