Table 2. Summary of affinity and kinetic... | American Society of Hematology

Table 2.

Summary of affinity and kinetic values for E-Ig binding to E-selLs: CD34, CD44, CD43, and PSGL-1 using a SPR-based assay

	CD34				CD44
	1st	2nd	3rd	Mean ± SEM	1st	2nd	3rd	Mean ± SEM
K_D, nM	145	268	297	236.7 ± 38	234	252	214	233 ± 9
k_off-apparent, s⁻¹	2.90E−04	1.60E−04	3.31E−04	2.6E−4 ± 0.4E−4	1.10E−04	2.00E−04	2.73E−04	1.9E−4 ± 0.4E−4
k_on-apparent, M⁻¹ s⁻¹	2030	597	1054	1230 ± 346	641	794	1280	903 ± 156
	CD43				PSGL-1
	1st	2nd		Mean ± SEM	1st	2nd	3rd	Mean ± SEM
K_D, nM	508.7	376		442 ± 47	342.5	207	229	259 ± 34
k_off-apparent, s⁻¹	4.80E−04	4.20E−04		4.5E−4 ± 0.2E−5	1.50E-04	1.65E−04	2.62E−04	1.9E−4 ± 0.3E−4
k_on-apparent, M⁻¹ s⁻¹	944	1063.8		1004 ± 43	438	797	1144	793 ± 166

	CD34				CD44
	1st	2nd	3rd	Mean ± SEM	1st	2nd	3rd	Mean ± SEM
K_D, nM	145	268	297	236.7 ± 38	234	252	214	233 ± 9
k_off-apparent, s⁻¹	2.90E−04	1.60E−04	3.31E−04	2.6E−4 ± 0.4E−4	1.10E−04	2.00E−04	2.73E−04	1.9E−4 ± 0.4E−4
k_on-apparent, M⁻¹ s⁻¹	2030	597	1054	1230 ± 346	641	794	1280	903 ± 156
	CD43				PSGL-1
	1st	2nd		Mean ± SEM	1st	2nd	3rd	Mean ± SEM
K_D, nM	508.7	376		442 ± 47	342.5	207	229	259 ± 34
k_off-apparent, s⁻¹	4.80E−04	4.20E−04		4.5E−4 ± 0.2E−5	1.50E-04	1.65E−04	2.62E−04	1.9E−4 ± 0.3E−4
k_on-apparent, M⁻¹ s⁻¹	944	1063.8		1004 ± 43	438	797	1144	793 ± 166