Table 3. Mixed-cell complement lysis:... | American Society of Hematology

Table 3.

Mixed-cell complement lysis: simulation study

Study RBC population	Sensitization*	6% albumin IgM	Anti-human IgG	Complement-mediated lysis†	Anti-human C3b/d
20% AET-treated	Group O plasma	W	2+^s	Weak hemolysis	0
	Pooled AB plasma	0	0	No hemolysis	0
PNH sample (9%)	Anti-I	1+	2+	Hemolysis	1+
	Pooled AB plasma	0	0	No hemolysis	0

Study RBC population	Sensitization*	6% albumin IgM	Anti-human IgG	Complement-mediated lysis†	Anti-human C3b/d
20% AET-treated	Group O plasma	W	2+^s	Weak hemolysis	0
	Pooled AB plasma	0	0	No hemolysis	0
PNH sample (9%)	Anti-I	1+	2+	Hemolysis	1+
	Pooled AB plasma	0	0	No hemolysis	0

Hemagglutination and hemolysis were scored according to Judd et al²⁰: 0, negative reaction, no agglutination; W, weak reaction, many tiny agglutinates; 1+, very small just visible agglutinates; 2+, many small visible agglutinates; 2+^s, medium sized visible agglutinates.

†Presence of hemolysis visualized after incubation with AB serum at 37°C for 60 min.

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