Table 1. Target proteins measured by MRM... | American Society of Hematology

Table 1.

Target proteins measured by MRM and their corresponding surrogate peptides

Protein	MRM peptide	Correlation between activity/antibody assay and MRM measurements
Protein	MRM peptide	Regression slope	Pearson correlation	R²	R² 95% confidence interval
α-2 antiplasmin	LGNQEPGGQTALK	0.68	0.68	0.46	0.3-0.62
α-2 antiplasmin	DFLQSLK	0.68	0.68	0.46	0.31-0.63
Antithrombin-III	DDLYVSDAFHK	0.60	0.67	0.45	0.25-0.66
	TSDQIHFFFAK	0.71	0.71	0.50	0.28-0.69
	FATTFYQHLADSK	0.62	0.62	0.38	0.2-0.6
Coagulation factor IX	IIPHHNYNAAINK	0.75	0.75	0.56	0.41-0.71
	SALVLQYLR	0.74	0.74	0.55	0.4-0.71
	VSVSQTSK	0.46	0.46	0.21	0.06-0.37
Coagulation factor V	GEYEEHLGILGPIIR	0.80	0.80	0.64	0.5-0.77
	AEVDDVIQVR	0.89	0.89	0.80	0.68-0.88
	SQHLDNFSNQIGK	0.86	0.86	0.74	0.61-0.86
Coagulation factor VII	VAQVIIPSTYVPGTTNHDIALLR	0.85	0.78	0.61	0.39-0.77
Coagulation factor X	ETYDFDIAVLR	1.08	0.74	0.55	0.4-0.68
	GYTLADNGK	1.24	0.86	0.73	0.66-0.84
	TGIVSGFGR	1.26	0.90	0.81	0.73-0.9
Coagulation factor XI	TSESGLPSTR	0.85	0.85	0.73	0.61-0.82
Fibrinogen α chain	ESSSHHPGIAEFPSR	0.68	0.68	0.46	0.25-0.65
	VQHIQLLQK	0.74	0.76	0.58	0.44-0.73
	GSESGIFTNTK	0.72	0.72	0.52	0.29-0.71
Fibrinogen β chain	AHYGGFTVQNEANK	0.79	0.79	0.62	0.43-0.77
	HQLYIDETVNSNIPTNLR	0.79	0.79	0.63	0.46-0.76
	YQISVNK	0.71	0.75	0.57	0.4-0.72
Fibrinogen γ chain	IHLISTQSAIPYALR	0.68	0.74	0.55	0.37-0.7
	YLQEIYNSNNQK	0.76	0.76	0.58	0.38-0.74
	YEASILTHDSSIR	0.80	0.80	0.64	0.47-0.78
Plasminogen	LFLEPTR	0.77	0.93	0.86	0.79-0.91
Prothrombin	ELLESYIDGR	1.34	0.87	0.76	0.64-0.85
	ETAASLLQAGYK	1.15	0.78	0.61	0.46-0.76
	TATSEYQTFFNPR	1.23	0.85	0.72	0.54-0.85
Vitamin K–dependent protein C	DTEDQEDQVDPR	0.88	0.88	0.77	0.66-0.87
	LGEYDLR	0.89	0.90	0.80	0.72-0.87
	TFVLNFIK	0.92	0.92	0.84	0.75-0.91
Vitamin K–dependent protein S	IETISHEDLQR	0.88	0.88	0.77	0.66-0.86
	SFQTGLFTAAR	0.86	0.86	0.74	0.6-0.85
	VYFAGFPR	0.90	0.90	0.81	0.69-0.89
VWF	ILAGPAGDSNVVK	0.11	0.18	0.03	0-0.26

Protein	MRM peptide	Correlation between activity/antibody assay and MRM measurements
Protein	MRM peptide	Regression slope	Pearson correlation	R²	R² 95% confidence interval
α-2 antiplasmin	LGNQEPGGQTALK	0.68	0.68	0.46	0.3-0.62
α-2 antiplasmin	DFLQSLK	0.68	0.68	0.46	0.31-0.63
Antithrombin-III	DDLYVSDAFHK	0.60	0.67	0.45	0.25-0.66
	TSDQIHFFFAK	0.71	0.71	0.50	0.28-0.69
	FATTFYQHLADSK	0.62	0.62	0.38	0.2-0.6
Coagulation factor IX	IIPHHNYNAAINK	0.75	0.75	0.56	0.41-0.71
	SALVLQYLR	0.74	0.74	0.55	0.4-0.71
	VSVSQTSK	0.46	0.46	0.21	0.06-0.37
Coagulation factor V	GEYEEHLGILGPIIR	0.80	0.80	0.64	0.5-0.77
	AEVDDVIQVR	0.89	0.89	0.80	0.68-0.88
	SQHLDNFSNQIGK	0.86	0.86	0.74	0.61-0.86
Coagulation factor VII	VAQVIIPSTYVPGTTNHDIALLR	0.85	0.78	0.61	0.39-0.77
Coagulation factor X	ETYDFDIAVLR	1.08	0.74	0.55	0.4-0.68
	GYTLADNGK	1.24	0.86	0.73	0.66-0.84
	TGIVSGFGR	1.26	0.90	0.81	0.73-0.9
Coagulation factor XI	TSESGLPSTR	0.85	0.85	0.73	0.61-0.82
Fibrinogen α chain	ESSSHHPGIAEFPSR	0.68	0.68	0.46	0.25-0.65
	VQHIQLLQK	0.74	0.76	0.58	0.44-0.73
	GSESGIFTNTK	0.72	0.72	0.52	0.29-0.71
Fibrinogen β chain	AHYGGFTVQNEANK	0.79	0.79	0.62	0.43-0.77
	HQLYIDETVNSNIPTNLR	0.79	0.79	0.63	0.46-0.76
	YQISVNK	0.71	0.75	0.57	0.4-0.72
Fibrinogen γ chain	IHLISTQSAIPYALR	0.68	0.74	0.55	0.37-0.7
	YLQEIYNSNNQK	0.76	0.76	0.58	0.38-0.74
	YEASILTHDSSIR	0.80	0.80	0.64	0.47-0.78
Plasminogen	LFLEPTR	0.77	0.93	0.86	0.79-0.91
Prothrombin	ELLESYIDGR	1.34	0.87	0.76	0.64-0.85
	ETAASLLQAGYK	1.15	0.78	0.61	0.46-0.76
	TATSEYQTFFNPR	1.23	0.85	0.72	0.54-0.85
Vitamin K–dependent protein C	DTEDQEDQVDPR	0.88	0.88	0.77	0.66-0.87
	LGEYDLR	0.89	0.90	0.80	0.72-0.87
	TFVLNFIK	0.92	0.92	0.84	0.75-0.91
Vitamin K–dependent protein S	IETISHEDLQR	0.88	0.88	0.77	0.66-0.86
	SFQTGLFTAAR	0.86	0.86	0.74	0.6-0.85
	VYFAGFPR	0.90	0.90	0.81	0.69-0.89
VWF	ILAGPAGDSNVVK	0.11	0.18	0.03	0-0.26

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