Table 1. Immunophenotyping panels Immune... | American Society of Hematology

Table 1.

Immunophenotypingpanels

Immune cell phenotype	Epitopes tested
T cells
CD3⁺ total	CD3⁺
CD4⁺ total	CD4⁺
CD8⁺ total	CD8⁺
T-cell subsets
CD3⁺CD4⁺	CD3⁺CD4⁺
CD4⁺CD8⁺ double positive	CD3⁺CD4⁺CD8⁺
CD4^–CD8^– double negative	CD3⁺CD4^–CD8^–
CD4⁺ naive	CD3⁺CD4⁺CD45RA⁺CD45RO^–CD27⁺
CD4⁺ central memory	CD3⁺CD4⁺CD45RA^–CD45RO⁺CD27⁺
CD4⁺ effector memory	CD3⁺CD4⁺CD45RA^–CD45RO⁺CD27^–
CD4⁺ effector	CD3⁺CD4⁺CD45RA⁺CD45RO^–CD27^–
CD4⁺ recent thymic emigrants	CD3⁺CD4⁺CD45RA⁺CD31⁺CD45RO^–
CD4^– recent thymic emigrants	CD3⁺CD4^–CD45RA⁺CD31⁺CD45RO^–
Tregs bright (CD4⁺)	CD3⁺CD4⁺CD25 (br)
Tregs dim (CD4⁺)	CD3⁺CD4⁺CD25⁺CD127 (d)
Tregs DR⁺ (CD4⁺)	CD3⁺CD4⁺CD25⁺CD127 (d)HLADr⁺
Tregs dim (CD4^–)	CD3⁺CD4^–CD25⁺CD127 (d)
Tregs DR^– (CD4^–)	CD3⁺CD4^–CD25⁺CD127^–HLADr^–
CD3⁺CD8⁺	CD3⁺CD8⁺
CD8⁺ naive	CD3⁺CD8⁺CD45RA⁺CD45RO^–CD27⁺
CD8⁺ primed (effector)	CD3⁺CD8⁺CD45RA⁺CD45RO^–CD27^–
CD8⁺ CM	CD3⁺CD8⁺CD45RA^–CD45RO⁺CD27⁺
CD8⁺ effector memory	CD3⁺CD8⁺CD45RA^–CD45RO⁺CD27^–
γδ T cells	CD3⁺ γδ⁺
B cells
CD19⁺ total	CD19⁺
CD20⁺ total	CD20⁺
CD19⁺ naive	CD19⁺CD27^–
Naive (Bm 1)	CD19⁺CD38^–IgD⁺CD27^–CD20^+/−
Naive (Bm 2)	CD19⁺CD38⁺IgD⁺CD27^–CD20^+/−
CD19⁺ memory	CD19⁺CD27⁺
Memory pre-switch	CD19⁺CD38^+/−IgD⁺CD27⁺CD20^+/−
Memory post-switch	CD19⁺CD38^+/−IgD^–CD27⁺CD20^+/−
NK cells
Total NK cells	CD56⁺CD16⁺
	CD16/56⁺ γδ^+/− CD8⁻ CD3⁺ CD4⁺
	CD16/56⁺ γδ^+/− CD8⁺ CD3⁺ CD4⁻
DCs
Myeloid CD11c⁺	HLADr⁺CD123^+/− Dump^– CD11c⁺
Plasmacytoid CD123⁺	HLADr⁺ CD123⁺ Dump^– CD11c^–

Immune cell phenotype	Epitopes tested
T cells
CD3⁺ total	CD3⁺
CD4⁺ total	CD4⁺
CD8⁺ total	CD8⁺
T-cell subsets
CD3⁺CD4⁺	CD3⁺CD4⁺
CD4⁺CD8⁺ double positive	CD3⁺CD4⁺CD8⁺
CD4^–CD8^– double negative	CD3⁺CD4^–CD8^–
CD4⁺ naive	CD3⁺CD4⁺CD45RA⁺CD45RO^–CD27⁺
CD4⁺ central memory	CD3⁺CD4⁺CD45RA^–CD45RO⁺CD27⁺
CD4⁺ effector memory	CD3⁺CD4⁺CD45RA^–CD45RO⁺CD27^–
CD4⁺ effector	CD3⁺CD4⁺CD45RA⁺CD45RO^–CD27^–
CD4⁺ recent thymic emigrants	CD3⁺CD4⁺CD45RA⁺CD31⁺CD45RO^–
CD4^– recent thymic emigrants	CD3⁺CD4^–CD45RA⁺CD31⁺CD45RO^–
Tregs bright (CD4⁺)	CD3⁺CD4⁺CD25 (br)
Tregs dim (CD4⁺)	CD3⁺CD4⁺CD25⁺CD127 (d)
Tregs DR⁺ (CD4⁺)	CD3⁺CD4⁺CD25⁺CD127 (d)HLADr⁺
Tregs dim (CD4^–)	CD3⁺CD4^–CD25⁺CD127 (d)
Tregs DR^– (CD4^–)	CD3⁺CD4^–CD25⁺CD127^–HLADr^–
CD3⁺CD8⁺	CD3⁺CD8⁺
CD8⁺ naive	CD3⁺CD8⁺CD45RA⁺CD45RO^–CD27⁺
CD8⁺ primed (effector)	CD3⁺CD8⁺CD45RA⁺CD45RO^–CD27^–
CD8⁺ CM	CD3⁺CD8⁺CD45RA^–CD45RO⁺CD27⁺
CD8⁺ effector memory	CD3⁺CD8⁺CD45RA^–CD45RO⁺CD27^–
γδ T cells	CD3⁺ γδ⁺
B cells
CD19⁺ total	CD19⁺
CD20⁺ total	CD20⁺
CD19⁺ naive	CD19⁺CD27^–
Naive (Bm 1)	CD19⁺CD38^–IgD⁺CD27^–CD20^+/−
Naive (Bm 2)	CD19⁺CD38⁺IgD⁺CD27^–CD20^+/−
CD19⁺ memory	CD19⁺CD27⁺
Memory pre-switch	CD19⁺CD38^+/−IgD⁺CD27⁺CD20^+/−
Memory post-switch	CD19⁺CD38^+/−IgD^–CD27⁺CD20^+/−
NK cells
Total NK cells	CD56⁺CD16⁺
	CD16/56⁺ γδ^+/− CD8⁻ CD3⁺ CD4⁺
	CD16/56⁺ γδ^+/− CD8⁺ CD3⁺ CD4⁻
DCs
Myeloid CD11c⁺	HLADr⁺CD123^+/− Dump^– CD11c⁺
Plasmacytoid CD123⁺	HLADr⁺ CD123⁺ Dump^– CD11c^–

Bm, B mature; br, bright; d, dim; DR, antigen D–related.

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