Table 2. Cellular content of the BM as... | American Society of Hematology

Table 2.

Cellular content of the BM as determined by flow cytometry

Populations	Humans			Mice
Populations	Phenotype	Frequency of BMMNC (%)	Reference(s)	Phenotype	Frequency of BMMNC (%)	Reference(s)
HSCs	Lin⁻CD34⁺CD38⁻CD90⁺CD45RA⁻	0.01-0.2	27, 28	Lin⁻c-kit⁺Sca-1⁺(LSK)		90
HSCs	Lin⁻CD34⁺CD38⁻CD90⁺CD45RA⁻	0.01-0.2	27, 28	CD48⁻CD150⁺	0.004-0.007	90
Common myeloid progenitors	Lin⁻CD34⁺CD38⁻CD123⁺CD45RA⁻	0.2-0.8	28, 91	Lin⁻c-kit⁺	0.4	92
Common myeloid progenitors	Lin⁻CD34⁺CD38⁻CD123⁺CD45RA⁻	0.2-0.8	28, 91	CD34⁺CD16/32^lo	0.4	92
Common lymphoid progenitors	Lin⁻CD34⁺CD38⁺	0.02-0.18	28	Lin⁻c-kit⁺CD127⁺	0.02	93
Common lymphoid progenitors	CD127⁺	0.02-0.18	28	Lin⁻c-kit⁺CD127⁺	0.02	93
Erythroid progenitors	CD235⁺CD71⁺	4-16	94	Ter119⁺	5-15	26, 95
Granulocytes	CD66⁺	57-86	94, 96	Gr-1⁺	30-50	26, 95
Megakaryocytes	*	0.05	22	CD41⁺	0.29	30
Monocytes	CD14⁺	2-5.8	94, 97	CD115⁺CD11b⁺Ly6C⁺	5-7	98
B-cell lineage (including progenitors)	CD19⁺	1-7	94, 96	B220	35-45	26
T lymphocytes	CD45⁺CD66⁻CD34⁻CD14⁻	8-20	94, 97	CD3⁺	4-5	26, 83
T lymphocytes	CD45⁺CD66⁻CD34⁻CD14⁻	8-20	94, 97	CD8⁺	2	26, 83
Endothelial cells	CD45⁻Ter119⁻CD31⁺	ND		CD45⁻Ter119⁻CD31⁺	0.03-0.4	32, 41, 99
Fibroblastic reticular stromal cells	CD146⁺	0.02-0.08	100-102	CD45⁻Ter119⁻CXCL12GFP⁺	0.02-0.04	31, 33
	CD271⁺	0.03		CD45⁻Ter119⁻CD140⁺LepR⁺	0.3
	CD146⁺CD51⁺CD140a⁺	0.1		CD45⁻Ter119⁻CD140⁺LepR⁺	0.3

Populations	Humans			Mice
Populations	Phenotype	Frequency of BMMNC (%)	Reference(s)	Phenotype	Frequency of BMMNC (%)	Reference(s)
HSCs	Lin⁻CD34⁺CD38⁻CD90⁺CD45RA⁻	0.01-0.2	27, 28	Lin⁻c-kit⁺Sca-1⁺(LSK)		90
HSCs	Lin⁻CD34⁺CD38⁻CD90⁺CD45RA⁻	0.01-0.2	27, 28	CD48⁻CD150⁺	0.004-0.007	90
Common myeloid progenitors	Lin⁻CD34⁺CD38⁻CD123⁺CD45RA⁻	0.2-0.8	28, 91	Lin⁻c-kit⁺	0.4	92
Common myeloid progenitors	Lin⁻CD34⁺CD38⁻CD123⁺CD45RA⁻	0.2-0.8	28, 91	CD34⁺CD16/32^lo	0.4	92
Common lymphoid progenitors	Lin⁻CD34⁺CD38⁺	0.02-0.18	28	Lin⁻c-kit⁺CD127⁺	0.02	93
Common lymphoid progenitors	CD127⁺	0.02-0.18	28	Lin⁻c-kit⁺CD127⁺	0.02	93
Erythroid progenitors	CD235⁺CD71⁺	4-16	94	Ter119⁺	5-15	26, 95
Granulocytes	CD66⁺	57-86	94, 96	Gr-1⁺	30-50	26, 95
Megakaryocytes	*	0.05	22	CD41⁺	0.29	30
Monocytes	CD14⁺	2-5.8	94, 97	CD115⁺CD11b⁺Ly6C⁺	5-7	98
B-cell lineage (including progenitors)	CD19⁺	1-7	94, 96	B220	35-45	26
T lymphocytes	CD45⁺CD66⁻CD34⁻CD14⁻	8-20	94, 97	CD3⁺	4-5	26, 83
T lymphocytes	CD45⁺CD66⁻CD34⁻CD14⁻	8-20	94, 97	CD8⁺	2	26, 83
Endothelial cells	CD45⁻Ter119⁻CD31⁺	ND		CD45⁻Ter119⁻CD31⁺	0.03-0.4	32, 41, 99
Fibroblastic reticular stromal cells	CD146⁺	0.02-0.08	100-102	CD45⁻Ter119⁻CXCL12GFP⁺	0.02-0.04	31, 33
	CD271⁺	0.03		CD45⁻Ter119⁻CD140⁺LepR⁺	0.3
	CD146⁺CD51⁺CD140a⁺	0.1		CD45⁻Ter119⁻CD140⁺LepR⁺	0.3

Estimated ranges of frequencies of different hematopoietic and nonhematopoietic populations in human and mouse BM expressed as percentages of BM mononuclear cells (BMMNCs).

Estimation based on microscopic examination of BM, not flow cytometry.

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