Genotyping and qRT-PCR primer sequences
| Target . | Forward . | Reverse . |
|---|---|---|
| Cre* | 5′- GTGTGGGACAGAGAACAAACC-3′ | 5′- ACATCTTCAGGTTCTGCGGG-3′ |
| T-cell receptor* | 5′- CAAATGTTGCTTGTCTGGTG-3′ | 5′- GTCAGTCGAGTGCACAGTTT-3′ |
| Heph† | 5′- ACACCTTTGTGACGGCCATC-3′ | 5′- CTCCACCCTGGATCATGAAGTC-3′ |
| CypA† | 5′- GGAGATGAATCTGTAGGACGAGTC-3′ | 5′- CTCCACCCTGGATCATGAAGTC-3′ |
| Tfr1† | 5′-CTCCATAGAGTTTGCTGACACCAT-3′ | 5′-TCCAGCCTCACGAGGAGTGTAT-3′ |
| Rps18† | 5′-TTCCAGCACATTTTGCGAGTA-3′ | 5′-CACGCCCTTAAGGCAGTGAT-3′ |
| Hepcidin† | 5′-CCTGAGCAGCACCACCACCTAT-3′ | 5′-AATGTCTGCCCTGCTTTCTTCCC-3′ |
| Target . | Forward . | Reverse . |
|---|---|---|
| Cre* | 5′- GTGTGGGACAGAGAACAAACC-3′ | 5′- ACATCTTCAGGTTCTGCGGG-3′ |
| T-cell receptor* | 5′- CAAATGTTGCTTGTCTGGTG-3′ | 5′- GTCAGTCGAGTGCACAGTTT-3′ |
| Heph† | 5′- ACACCTTTGTGACGGCCATC-3′ | 5′- CTCCACCCTGGATCATGAAGTC-3′ |
| CypA† | 5′- GGAGATGAATCTGTAGGACGAGTC-3′ | 5′- CTCCACCCTGGATCATGAAGTC-3′ |
| Tfr1† | 5′-CTCCATAGAGTTTGCTGACACCAT-3′ | 5′-TCCAGCCTCACGAGGAGTGTAT-3′ |
| Rps18† | 5′-TTCCAGCACATTTTGCGAGTA-3′ | 5′-CACGCCCTTAAGGCAGTGAT-3′ |
| Hepcidin† | 5′-CCTGAGCAGCACCACCACCTAT-3′ | 5′-AATGTCTGCCCTGCTTTCTTCCC-3′ |