Table 1. Antibody panel for cell sorting... | American Society of Hematology

Table 1.

Antibody panel for cell sorting and phenotyping by flow cytometry

Antigen	Fluorophore	Clone	Supplier	Concentration (μL/1e6 cells)
CD45	V500	HI30	BD Biosciences	5
CRTH2	Alexa Fluor 647	BM16	BioLegend	5
CD117	PECy7	104D2	eBioscience	5
CD127	BV421	HIL-7R-M21	BD Biosciences	5
NKp44	PE	P44-8	BioLegend	5
Lineage panel
CD11b	FITC	ICRF44	BioLegend	5
CD14	FITC	M5E2	BD Biosciences	0.61
CD16	FITC	abioCB16	BD Biosciences	5
CD303a	FITC	201A	BioLegend	5
TCRab	FITC	IP26	BioLegend	2.5
CD19	FITC	HIB19	BioLegend	0.31
CD123	FITC	6H6	BioLegend	5
CD34	FITC	581	BioLegend	0.55
FcERI	FITC	AER-37	BioLegend	5
CD1a	FITC	HI149	eBioscience	0.17
CD94	FITC	HP-3D9	BD Biosciences	5
CD3	FITC	UCHT1	BioLegend	1.67
CD20	FITC	L27	BD Biosciences	1.67
CD11c	FITC	3.9	eBioscience	5
TCRgd	FITC	B1	BioLegend	5
Brilliant stain buffer	n/a	n/a	BD Biosciences	5
Antibodies used for cell phenotyping
CD117	BV650	104D2	BD Biosciences	5
CRTH2	PE	BM16	eBioscience	5
TSLPR	PerCP-efluor710	eBio1A6	eBioscience	2.5
IL-17BR	Unconjugated	D9.2	n/a	1.25
T1/ST2	FITC	B4E6	MD Biosciences	2.5
HLA-DR	APCCy7	G46-6	BD Biosciences	5
Gata 3	BV711	L50-823	BD Biosciences	2.5
Rorgt	BV650	Q21-559	BD Biosciences	5
T-bet	PECy7	4B10	BioLegend	5
CD117	PE	104D2	BioLegend	5
CD161	APCCy7	HP-3G10	BioLegend	5
NKp30	BV605	p30-15	BD Horizon	5
NKG2D	PerCpCy5.5	1D11	BioLegend	5
IL-13	BV711	JES10-5A2	BD Biosciences	2.5
IL-5	PE	JES1-39D10	BioLegend	2.5
IL-22	PerCPefluor710	22URTI	eBioscience	3
IL-17A	BV605	BL168	BioLegend	2.5
IFN-γ	APCefluor780	4S.B3	eBioscience	3
Granzyme B	FITC	GB11	BioLegend	2.5
Perforin	APCCy7	dG9	BioLegend	2.5
CRTH2	PEDazzle	BM16	BioLegend	5

Antigen	Fluorophore	Clone	Supplier	Concentration (μL/1e6 cells)
CD45	V500	HI30	BD Biosciences	5
CRTH2	Alexa Fluor 647	BM16	BioLegend	5
CD117	PECy7	104D2	eBioscience	5
CD127	BV421	HIL-7R-M21	BD Biosciences	5
NKp44	PE	P44-8	BioLegend	5
Lineage panel
CD11b	FITC	ICRF44	BioLegend	5
CD14	FITC	M5E2	BD Biosciences	0.61
CD16	FITC	abioCB16	BD Biosciences	5
CD303a	FITC	201A	BioLegend	5
TCRab	FITC	IP26	BioLegend	2.5
CD19	FITC	HIB19	BioLegend	0.31
CD123	FITC	6H6	BioLegend	5
CD34	FITC	581	BioLegend	0.55
FcERI	FITC	AER-37	BioLegend	5
CD1a	FITC	HI149	eBioscience	0.17
CD94	FITC	HP-3D9	BD Biosciences	5
CD3	FITC	UCHT1	BioLegend	1.67
CD20	FITC	L27	BD Biosciences	1.67
CD11c	FITC	3.9	eBioscience	5
TCRgd	FITC	B1	BioLegend	5
Brilliant stain buffer	n/a	n/a	BD Biosciences	5
Antibodies used for cell phenotyping
CD117	BV650	104D2	BD Biosciences	5
CRTH2	PE	BM16	eBioscience	5
TSLPR	PerCP-efluor710	eBio1A6	eBioscience	2.5
IL-17BR	Unconjugated	D9.2	n/a	1.25
T1/ST2	FITC	B4E6	MD Biosciences	2.5
HLA-DR	APCCy7	G46-6	BD Biosciences	5
Gata 3	BV711	L50-823	BD Biosciences	2.5
Rorgt	BV650	Q21-559	BD Biosciences	5
T-bet	PECy7	4B10	BioLegend	5
CD117	PE	104D2	BioLegend	5
CD161	APCCy7	HP-3G10	BioLegend	5
NKp30	BV605	p30-15	BD Horizon	5
NKG2D	PerCpCy5.5	1D11	BioLegend	5
IL-13	BV711	JES10-5A2	BD Biosciences	2.5
IL-5	PE	JES1-39D10	BioLegend	2.5
IL-22	PerCPefluor710	22URTI	eBioscience	3
IL-17A	BV605	BL168	BioLegend	2.5
IFN-γ	APCefluor780	4S.B3	eBioscience	3
Granzyme B	FITC	GB11	BioLegend	2.5
Perforin	APCCy7	dG9	BioLegend	2.5
CRTH2	PEDazzle	BM16	BioLegend	5

FITC, fluorescein isothiocyanate; n/a, not applicable; PE, phycoerythrin.