Table 1 Combination treatment with low... | American Society of Hematology

Table 1

Combination treatment with low doses of RG7112 and Peg-IFNα 2a reduced the number of JAKV617F⁺ hematopoietic colonies

Patient number*	JAK2 V617F allele burden (%)	Total colonies genotyped	Additions to culture
			Cytokines only (control)			Peg-IFNα 2a 200 ng/mL + RG7112 200 nM
			Homo %	Hetero %	WT† %	Homo %	Hetero %	WT† %
2	14	127	8.3	33.3	58	11.7	17.6	70.6
15	70	129	76.2	9.5	14.3	55	5	40
5	49	144	21	9	70	21	0	79
6	22	132	50	14	38	29	21	50
13	75	180	25	54	21	20	30	50
14	20	168	30	39	31	15	40	45
3	40	189	17	4	79	11	2	87
16	50	121	44	50	4	58	18	24
22	70	121	59	35	6	64	9	27
21	45	112	4.5	45.5	50	0	16.7	83.3‡
19	+	105	12.5	75	12.5	9.1	0	91‡

Patient number*	JAK2 V617F allele burden (%)	Total colonies genotyped	Additions to culture
			Cytokines only (control)			Peg-IFNα 2a 200 ng/mL + RG7112 200 nM
			Homo %	Hetero %	WT† %	Homo %	Hetero %	WT† %
2	14	127	8.3	33.3	58	11.7	17.6	70.6
15	70	129	76.2	9.5	14.3	55	5	40
5	49	144	21	9	70	21	0	79
6	22	132	50	14	38	29	21	50
13	75	180	25	54	21	20	30	50
14	20	168	30	39	31	15	40	45
3	40	189	17	4	79	11	2	87
16	50	121	44	50	4	58	18	24
22	70	121	59	35	6	64	9	27
21	45	112	4.5	45.5	50	0	16.7	83.3‡
19	+	105	12.5	75	12.5	9.1	0	91‡

24 to 48 colonies were plucked and examined in each condition. Homo, homozygous; hetero, heterozygous.

The number indicates the individual patients listed in supplemental Table 2.

†

A 1-tailed paired Student t test was used for statistical analysis. Every individual case was compared with treatment with cytokines alone, and all cases were included in the statistical analysis; P = .019.

‡

Peg-IFNα 2a 200 ng/ mL + RG7112 100 nM.

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