Kinetic parameters of FXIII-A2B2 complex formation
| . | n . | ka ± SE (1/Ms × 105) . | kd ± SE (1/s × 10−4) . | Kd ± SE (M × 10−10) . |
|---|---|---|---|---|
| SPR assay | ||||
| cFXIII-A2 + FXIII-B2 | 7 | 6.35 ± 0.80 | 2.62 ± 0.30 | 5.17 ± 1.44 |
| rFXIIII-A2 + FXIII-B2 | 4 | 5.76 ± 0.79 | 1.69 ± 0.08 | 3.14 ± 0.55 |
| rFXIII-A2 + rFXIII-B2 | 3 | 1.38 ± 0.30 | 0.76 ± 0.26 | 6.26 ± 2.76 |
| ELISA-type assay | ||||
| cFXIII-A2 + FXIII-B2 | 4 | — | — | 2.93 ± 0.68 |
| rFXIIII-A2 + FXIII-B2 | 3 | — | — | 5.43 ± 0.95 |
| . | n . | ka ± SE (1/Ms × 105) . | kd ± SE (1/s × 10−4) . | Kd ± SE (M × 10−10) . |
|---|---|---|---|---|
| SPR assay | ||||
| cFXIII-A2 + FXIII-B2 | 7 | 6.35 ± 0.80 | 2.62 ± 0.30 | 5.17 ± 1.44 |
| rFXIIII-A2 + FXIII-B2 | 4 | 5.76 ± 0.79 | 1.69 ± 0.08 | 3.14 ± 0.55 |
| rFXIII-A2 + rFXIII-B2 | 3 | 1.38 ± 0.30 | 0.76 ± 0.26 | 6.26 ± 2.76 |
| ELISA-type assay | ||||
| cFXIII-A2 + FXIII-B2 | 4 | — | — | 2.93 ± 0.68 |
| rFXIIII-A2 + FXIII-B2 | 3 | — | — | 5.43 ± 0.95 |
Results are expressed as mean ± standard error (SE).
rFXIII-B2, recombinant FXIII-B2.