Primer pairs used to PCR amplify FOXP1 isoforms and product sizes
Primer pair . | Isoform no. and PCR product size . | Isoform no. and smaller PCR product size . |
---|---|---|
PJB017/PJB018 | Isoform 1 = 556 bp | Isoform 3 = 328 bp |
PJB019/PJB018 | Isoform 4 = 483 bp | Isoform 5 = 255 bp |
PJB020/PJB021 | Isoform 9 = 535 bp | Isoform 8 = 271 bp |
PJB045/PJB038 | Isoform 3 = 305 bp | — |
Actin for/rev | Actin = 837 bp | — |
Primer pair . | Isoform no. and PCR product size . | Isoform no. and smaller PCR product size . |
---|---|---|
PJB017/PJB018 | Isoform 1 = 556 bp | Isoform 3 = 328 bp |
PJB019/PJB018 | Isoform 4 = 483 bp | Isoform 5 = 255 bp |
PJB020/PJB021 | Isoform 9 = 535 bp | Isoform 8 = 271 bp |
PJB045/PJB038 | Isoform 3 = 305 bp | — |
Actin for/rev | Actin = 837 bp | — |
— indicates not applicable.