Individual experiments showing culture wells with different Delta1ext-IgG densities containing (+) or lacking (-) B-cell progenitors defined as cells capable of generating B220+CD19+ cells following transfer to an OP-9 stromal layer
| Genotype . | Delta1ext-IgG plating concentration . | No. positive higher-density Delta1ext-IgG wells from Hes5−/− LSKSP compared with Hes5+/+ LSKSP . | |||||
|---|---|---|---|---|---|---|---|
| 0 μg/mL . | Low . | Intermediate . | High . | ||||
| 0.6 μg/mL . | 1.25 μg/mL . | 2.5 μg/mL . | 5.0 μg/mL . | 10.0 μg/mL . | |||
| Experiment 1 | 2 | ||||||
| Hes5+/+ | + | + | − | − | − | − | |
| Hes5−/− | − | + | + | + | − | − | |
| Experiment 2 | − | 1 | |||||
| Hes5+/+ | − | + | + | + | − | − | |
| Hes5−/− | − | + | + | + | + | − | |
| Experiment 3 | − | 0 | |||||
| Hes5+/+ | − | + | + | − | − | − | |
| Hes5−/− | − | + | + | − | − | − | |
| Experiment 4 | 1 | ||||||
| Hes5+/+ | − | + | − | − | − | − | |
| Hes5−/− | + | + | + | − | − | − | |
| Experiment 5 | 2 | ||||||
| Hes5+/+ | + | − | + | − | − | − | |
| Hes5−/− | − | − | + | + | + | − | |
| Total no. positive | 6 | ||||||
| Hes5+/+ | 2 | 5 | 3 | 1 | 0 | 0 | |
| Hes5−/− | 1 | 5 | 5 | 3 | 2 | 0 | |
| Genotype . | Delta1ext-IgG plating concentration . | No. positive higher-density Delta1ext-IgG wells from Hes5−/− LSKSP compared with Hes5+/+ LSKSP . | |||||
|---|---|---|---|---|---|---|---|
| 0 μg/mL . | Low . | Intermediate . | High . | ||||
| 0.6 μg/mL . | 1.25 μg/mL . | 2.5 μg/mL . | 5.0 μg/mL . | 10.0 μg/mL . | |||
| Experiment 1 | 2 | ||||||
| Hes5+/+ | + | + | − | − | − | − | |
| Hes5−/− | − | + | + | + | − | − | |
| Experiment 2 | − | 1 | |||||
| Hes5+/+ | − | + | + | + | − | − | |
| Hes5−/− | − | + | + | + | + | − | |
| Experiment 3 | − | 0 | |||||
| Hes5+/+ | − | + | + | − | − | − | |
| Hes5−/− | − | + | + | − | − | − | |
| Experiment 4 | 1 | ||||||
| Hes5+/+ | − | + | − | − | − | − | |
| Hes5−/− | + | + | + | − | − | − | |
| Experiment 5 | 2 | ||||||
| Hes5+/+ | + | − | + | − | − | − | |
| Hes5−/− | − | − | + | + | + | − | |
| Total no. positive | 6 | ||||||
| Hes5+/+ | 2 | 5 | 3 | 1 | 0 | 0 | |
| Hes5−/− | 1 | 5 | 5 | 3 | 2 | 0 | |