List of peptides down-regulated more than 2-fold in NB4 cells treated with DNR (1.6 μmol/L) and CHX (3.6 μmol/L) for 8 hours
| Protein description . | Accession no. . | Start . | End . | Identified peptide . | Fold down-regulation . |
|---|---|---|---|---|---|
| EF2 kinase | O00418 | 2 | 9 | Ac-ADEDLIFR | >10 |
| Lysyl-tRNA synthetase | Q15046 | 2 | 25 | Ac-AAVQAAEVKVDGSEPKLSKNELKR | >10 |
| eIF4H, isoform 1 | Q15056 | 2 | 10 | Ac-ADFDTYDDR | >10 |
| 60S ribosomal protein L13 | P40429 | 2 | 12 | Ac-AEVQVLVLDGR | 5.9 |
| Asparaginyl-tRNA synthetase, cytoplasmic | O43776 | 2 | 11 | Ac-VLAELYVSDR | 4.2 |
| hnRNP C1/C2, isoform 1 | P07910 | 2 | 12 | Ac-ASNVTN<Dam>KTDPR | 4.4 |
| Ac-ASNVTNKTDPR | 2.9 | ||||
| 40S ribosomal protein S30 | P62861 | 1 | 8 | Ac-KVHGSLAR | 2.5 |
| 60S ribosomal protein L30 | P62888 | 2 | 17 | Ac-VAAKKTKKSLESINSR | 2.5 |
| LSm3 | P62310 | 2 | 22 | Ac-ADDVDQQQTTNTVEEPLDLIR | 2.5 |
| eEF2 | P13639 | 2 | 10 | Ac-VN<Dam>FTVDQIR | 2.4 |
| eIF1A | O14602 | 2 | 12 | Ac-PKNKGKGGKNR | 2.3 |
| Phenylalanyl-tRNA synthetase alpha chain | Q9Y285 | 2 | 12 | Ac-ADGQVAELLLR | 2.3 |
| 40S ribosomal protein S3a | P61247 | 2 | 8 | Ac-AVGKN<Dam>KR | 2.2 |
| FK506-binding protein 1A/FKBP 12 | P62942 | 2 | 14 | Ac-GVQVETISPGDGR | 2.1 |
| Protein description . | Accession no. . | Start . | End . | Identified peptide . | Fold down-regulation . |
|---|---|---|---|---|---|
| EF2 kinase | O00418 | 2 | 9 | Ac-ADEDLIFR | >10 |
| Lysyl-tRNA synthetase | Q15046 | 2 | 25 | Ac-AAVQAAEVKVDGSEPKLSKNELKR | >10 |
| eIF4H, isoform 1 | Q15056 | 2 | 10 | Ac-ADFDTYDDR | >10 |
| 60S ribosomal protein L13 | P40429 | 2 | 12 | Ac-AEVQVLVLDGR | 5.9 |
| Asparaginyl-tRNA synthetase, cytoplasmic | O43776 | 2 | 11 | Ac-VLAELYVSDR | 4.2 |
| hnRNP C1/C2, isoform 1 | P07910 | 2 | 12 | Ac-ASNVTN<Dam>KTDPR | 4.4 |
| Ac-ASNVTNKTDPR | 2.9 | ||||
| 40S ribosomal protein S30 | P62861 | 1 | 8 | Ac-KVHGSLAR | 2.5 |
| 60S ribosomal protein L30 | P62888 | 2 | 17 | Ac-VAAKKTKKSLESINSR | 2.5 |
| LSm3 | P62310 | 2 | 22 | Ac-ADDVDQQQTTNTVEEPLDLIR | 2.5 |
| eEF2 | P13639 | 2 | 10 | Ac-VN<Dam>FTVDQIR | 2.4 |
| eIF1A | O14602 | 2 | 12 | Ac-PKNKGKGGKNR | 2.3 |
| Phenylalanyl-tRNA synthetase alpha chain | Q9Y285 | 2 | 12 | Ac-ADGQVAELLLR | 2.3 |
| 40S ribosomal protein S3a | P61247 | 2 | 8 | Ac-AVGKN<Dam>KR | 2.2 |
| FK506-binding protein 1A/FKBP 12 | P62942 | 2 | 14 | Ac-GVQVETISPGDGR | 2.1 |
In Tables 2 and 3, only peptides with corresponding parent proteins with functions related to mRNA-binding/processing and/or protein synthesis are shown. The sequence of the identified peptide and its location within the parent protein is indicated Ac- denotes the α-N-acetyl group, and <Dam> indicates deamidation. The parent proteins are referred to by description and UnitProt database accession number, The complete list of peptides is given in Table S1. Peptide identification was done using “Internal Protein Index” databases (http://www.ebi.ac.uk/IPI/IPIhelp.html).