Evaluation of CAR receptor expression on EBV-CTL after stimulation with EBV+ and CD30+ tumor cells
| CD30CAR+ cells . | Stimulation . | ||
|---|---|---|---|
| Second . | Third . | Fourth . | |
| After stimulation with EBV+ tumor cells | 19 ± 10 | 23.8 ± 5 | 16.8 ± 8 |
| After stimulation with CD30+ tumor cells | 18.2 ± 14 | 26.4 ± 10 | 21 ± 11 |
| CD30CAR+ cells . | Stimulation . | ||
|---|---|---|---|
| Second . | Third . | Fourth . | |
| After stimulation with EBV+ tumor cells | 19 ± 10 | 23.8 ± 5 | 16.8 ± 8 |
| After stimulation with CD30+ tumor cells | 18.2 ± 14 | 26.4 ± 10 | 21 ± 11 |
Data are percentages of CD30CAR+ cells. Evaluation was of CD30CAR expression on transduced CTLs after the indicated stimulation with autologous LCLs or CD30+ tumor cells. Analysis was performed by FACS after staining with antihuman IgG (H+L) Ab.