Table 3 Stem and progenitor cell... | American Society of Hematology

Table 3

Stem and progenitor cell analyses of frequency and cell-cycle status

BM source	Subpopulation	Stem cells			Progenitors
BM source	Subpopulation	Phenotype	Frequency, %	Cycle, %	Phenotype	Frequency, %	Cycle, %	CFU/25K
Naive control	NA	Sca⁺/Kit⁺/Lin⁻	0.08	10	Kit⁺/Lin⁻	1.70	27	30
GFP control	GFP⁺	Sca⁺/Kit⁺/Lin⁻	0.02	14	Kit⁺/Lin⁻	1.50	35	nd
GFP control	GFP⁻	Sca⁺/Kit⁺/Lin⁻	0.03	14	Kit⁺/Lin⁻	1.90	34	nd
BCR only	GFP⁺	Sca⁺/Kit⁺/Lin⁻	0.02	32	Kit⁺/Lin⁻	1.90	43	48
BCR only	GFP⁻	Sca⁺/Kit⁺/Lin⁻	0.04	17	Kit⁺/Lin⁻	1.60	25	nd
BCR + Nup	GFP⁺	Sca⁺/Flt⁺/Lin⁻*	0.30	10	Kit⁺/Lin⁻	8.40	28	442
BCR + Nup	GFP⁻	Sca⁺/Kit⁺/Lin⁻	0.07	13	Kit⁺/Lin⁻	1.90	21	nd

BM source	Subpopulation	Stem cells			Progenitors
BM source	Subpopulation	Phenotype	Frequency, %	Cycle, %	Phenotype	Frequency, %	Cycle, %	CFU/25K
Naive control	NA	Sca⁺/Kit⁺/Lin⁻	0.08	10	Kit⁺/Lin⁻	1.70	27	30
GFP control	GFP⁺	Sca⁺/Kit⁺/Lin⁻	0.02	14	Kit⁺/Lin⁻	1.50	35	nd
GFP control	GFP⁻	Sca⁺/Kit⁺/Lin⁻	0.03	14	Kit⁺/Lin⁻	1.90	34	nd
BCR only	GFP⁺	Sca⁺/Kit⁺/Lin⁻	0.02	32	Kit⁺/Lin⁻	1.90	43	48
BCR only	GFP⁻	Sca⁺/Kit⁺/Lin⁻	0.04	17	Kit⁺/Lin⁻	1.60	25	nd
BCR + Nup	GFP⁺	Sca⁺/Flt⁺/Lin⁻*	0.30	10	Kit⁺/Lin⁻	8.40	28	442
BCR + Nup	GFP⁻	Sca⁺/Kit⁺/Lin⁻	0.07	13	Kit⁺/Lin⁻	1.90	21	nd

Frequency indicates percentage of stem or progenitors cells as defined by the indicated phenotype; cycle %, percentage of cells in S + G2 phase of cell cycle; and CFU/25K, no. of colony-forming units per 25 000 cells plated in methylcellulose assay.

LSC phenotype (Sca⁺, Flt3⁺, kit⁻lo, CD34⁺, CD150⁻).

nd indicates not done.

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