Enumeration of total cells and lymphocyte populations in various tissues of wild-type and Dok-3−/− mice
| Tissue . | Total cells, × 107 . | CD3+, × 106 . | B220+ IgM+, × 106 . | CD5+ IgM+, × 106 . | B220+ IgM−, × 106 . | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| +/+ . | −/− . | +/+ . | −/− . | +/+ . | −/− . | +/+ . | −/− . | +/+ . | −/− . | |
| Spleen | 10.32 ± 1.13 n=6 | 10.83 ± 0.88 n=6 | 23.00 ± 4.20 n=6 | 27.30 ± 5.00 n=6 | 18.30 ± 2.00 n=6 | 21.50 ± 1.50 n=6 | ND | ND | ND | ND |
| Bone marrow | 3.78 ± 0.58 n=6 | 3.46 ± 0.41 n=6 | ND | ND | 1.50 ± 0.30 n=6 | 1.60 ± 0.20 n=6 | ND | ND | 1.90 ± 0.50 n=6 | 2.50 ± 0.60 n=6 |
| Peritoneum | 0.39 ± 0.03 n=10 | 0.45 ± 0.07 n=9 | ND | ND | 1.08 ± 0.22 n=6 | 0.68 ± 0.11 n=7 | 0.40 ± 0.05 n=7 | 0.40 ± 0.06 n=7 | ND | ND |
| Lymph node | 1.10 ± 0.40 n=4 | 1.15 ± 0.39 n=4 | 4.37 ± 1.43 n=4 | 4.25 ± 1.19 n=4 | 1.60 ± 0.73 n=4 | 1.65 ± 0.71 n=4 | ND | ND | ND | ND |
| Tissue . | Total cells, × 107 . | CD3+, × 106 . | B220+ IgM+, × 106 . | CD5+ IgM+, × 106 . | B220+ IgM−, × 106 . | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| +/+ . | −/− . | +/+ . | −/− . | +/+ . | −/− . | +/+ . | −/− . | +/+ . | −/− . | |
| Spleen | 10.32 ± 1.13 n=6 | 10.83 ± 0.88 n=6 | 23.00 ± 4.20 n=6 | 27.30 ± 5.00 n=6 | 18.30 ± 2.00 n=6 | 21.50 ± 1.50 n=6 | ND | ND | ND | ND |
| Bone marrow | 3.78 ± 0.58 n=6 | 3.46 ± 0.41 n=6 | ND | ND | 1.50 ± 0.30 n=6 | 1.60 ± 0.20 n=6 | ND | ND | 1.90 ± 0.50 n=6 | 2.50 ± 0.60 n=6 |
| Peritoneum | 0.39 ± 0.03 n=10 | 0.45 ± 0.07 n=9 | ND | ND | 1.08 ± 0.22 n=6 | 0.68 ± 0.11 n=7 | 0.40 ± 0.05 n=7 | 0.40 ± 0.06 n=7 | ND | ND |
| Lymph node | 1.10 ± 0.40 n=4 | 1.15 ± 0.39 n=4 | 4.37 ± 1.43 n=4 | 4.25 ± 1.19 n=4 | 1.60 ± 0.73 n=4 | 1.65 ± 0.71 n=4 | ND | ND | ND | ND |
Cell counts were calculated based on total cell numbers and percentage of the different lymphocyte populations as determined by FACS staining. Cell numbers are presented as mean ± SEM. Statistical significance was determined by paired 2-tailed Student t test.
n indicates the sample size; ND, not determined.