Primers used in the semi-quantitative PCR studies
| Gene and position . | Sequences . | Length, bp . |
|---|---|---|
| PRDM1α | ||
| Promoter | 721 | |
| PRDM1α-1F | 5′-TCCTCTGAACTGTGAAACGAC-3′ | |
| PRDM1α-1R | 5′-GTGTCACGGCAGCACTTTGTC-3′ | |
| Promoter | 981 | |
| PRDM1α-2F | 5′-TCTGAGGACTCTTAGGAATTG-3′ | |
| PRDM1α-2R | 5′-TTCCTCTCCTCCCAGAACCCA-3′ | |
| Exon 2/intron 2 junction | 366 | |
| PRDM1α-3F | 5′-AGAAGGAGCCACAGGAACG-3′ | |
| PRDM1α-3R | 5′-AGTAGGGAGATTTGGCACC-3′ | |
| Exon 2–exon 3 | 239 | |
| PRDM1α-4F | 5′-GTTCCTAAGAACGCCAACAGG-3′ | |
| PRDM1α-4R | 5′-GCAAAGTCCCGACAATACCAC-3′ | |
| Exon 2–exon 4 | 354 | |
| PRDM1α-5F | 5′-GGACATGGAGGATGCGGATAT-3′ | |
| PRDM1α-5R | 5′-GTTGCTTTTCTCTTCATTAAAGCCG-3′ | |
| Exon 3 | 463 | |
| PRDM1α-6F | 5′-GCTTGGTTGAGTATTTGCT-3′ | |
| PRDM1α-6R | 5′-GCTGGATACTTATGGGTGA-3′ | |
| Exon 4 | 382 | |
| PRDM1α-7F | 5′-CGGCTGTGTTTATTCTGAG-3′ | |
| PRDM1α-7R | 5′-GAACCGACATTACTGGCAT-3′ | |
| Exon 5 | 489 | |
| PRDM1α-8F | 5′-TTTGGCAGTTTTGCTTCAG-3′ | |
| PRDM1α-8R | 5′-CACAGGGGACACCGTATTC-3′ | |
| Exon 5 | 540 | |
| PRDM1α-9F | 5′-GACCCACGTACATCACTCGC-3′ | |
| PRDM1α-9R | 5′-CGGTAAAGGAGAAGGCACTG-3′ | |
| Exon 5 | 487 | |
| PRDM1α-10F | 5′-GTCTACAGCAATCTCCTCG-3′ | |
| PRDM1α-10R | 5′-CACAAGCATGCACTCACAG-3′ | |
| Exon 5–exon 7 | 471 | |
| PRDM1α-11F | 5′-AGTACGAATGCAACGTTTGCG-3′ | |
| PRDM1α-11R | 5′-TCGATTTCTTCATTGATTCGGGT-3′ | |
| Exon 6 | 292 | |
| PRDM1α-12F | 5′-GGAGCAGAAATGTTAGGTC-3′ | |
| PRDM1α-12R | 5′-GTGTTGGCTTTAACTACGG-3′ | |
| Exon 7 | 413 | |
| PRDM1α-13F | 5′-CCGTTGGCAACTCTTAATC-3′ | |
| PRDM1α-13R | 5′-TGTCATCCTCCACGTCCTC-3′ | |
| Exon 7 | 494 | |
| PRDM1α-14F | 5′-CACAAGAACTACATCCATC-3′ | |
| PRDM1α-14R | 5′-CAGAGCTGGGATTATGTAC-3′ | |
| PRDM1β | ||
| Intron 3–exon 4 | 264 | |
| PRDM1β-F | 5′-TGGTGGGTTAATCGGTTTGAG-3′ | |
| PRDM1β-R | 5′-GGGATGGGCTTAATGGTGTAG-3′ | |
| IRF4 | ||
| Exon 2–exon 3 | 114 | |
| IRF4-F | 5′-AGAACGAGGAGAAGAGCATC-3′ | |
| IRF4-R | 5′-CCTTTAAACAGTGCCCAAG-3′ | |
| GAPDH | ||
| Exon 1–exon 2 | 255 | |
| GAPDH-F | 5′-GAAGGTGAAGGTCGGAGTC-3′ | |
| GAPDH-R | 5′-GAAGATGGTGATGGGATTTC-3′ | |
| Gene and position . | Sequences . | Length, bp . |
|---|---|---|
| PRDM1α | ||
| Promoter | 721 | |
| PRDM1α-1F | 5′-TCCTCTGAACTGTGAAACGAC-3′ | |
| PRDM1α-1R | 5′-GTGTCACGGCAGCACTTTGTC-3′ | |
| Promoter | 981 | |
| PRDM1α-2F | 5′-TCTGAGGACTCTTAGGAATTG-3′ | |
| PRDM1α-2R | 5′-TTCCTCTCCTCCCAGAACCCA-3′ | |
| Exon 2/intron 2 junction | 366 | |
| PRDM1α-3F | 5′-AGAAGGAGCCACAGGAACG-3′ | |
| PRDM1α-3R | 5′-AGTAGGGAGATTTGGCACC-3′ | |
| Exon 2–exon 3 | 239 | |
| PRDM1α-4F | 5′-GTTCCTAAGAACGCCAACAGG-3′ | |
| PRDM1α-4R | 5′-GCAAAGTCCCGACAATACCAC-3′ | |
| Exon 2–exon 4 | 354 | |
| PRDM1α-5F | 5′-GGACATGGAGGATGCGGATAT-3′ | |
| PRDM1α-5R | 5′-GTTGCTTTTCTCTTCATTAAAGCCG-3′ | |
| Exon 3 | 463 | |
| PRDM1α-6F | 5′-GCTTGGTTGAGTATTTGCT-3′ | |
| PRDM1α-6R | 5′-GCTGGATACTTATGGGTGA-3′ | |
| Exon 4 | 382 | |
| PRDM1α-7F | 5′-CGGCTGTGTTTATTCTGAG-3′ | |
| PRDM1α-7R | 5′-GAACCGACATTACTGGCAT-3′ | |
| Exon 5 | 489 | |
| PRDM1α-8F | 5′-TTTGGCAGTTTTGCTTCAG-3′ | |
| PRDM1α-8R | 5′-CACAGGGGACACCGTATTC-3′ | |
| Exon 5 | 540 | |
| PRDM1α-9F | 5′-GACCCACGTACATCACTCGC-3′ | |
| PRDM1α-9R | 5′-CGGTAAAGGAGAAGGCACTG-3′ | |
| Exon 5 | 487 | |
| PRDM1α-10F | 5′-GTCTACAGCAATCTCCTCG-3′ | |
| PRDM1α-10R | 5′-CACAAGCATGCACTCACAG-3′ | |
| Exon 5–exon 7 | 471 | |
| PRDM1α-11F | 5′-AGTACGAATGCAACGTTTGCG-3′ | |
| PRDM1α-11R | 5′-TCGATTTCTTCATTGATTCGGGT-3′ | |
| Exon 6 | 292 | |
| PRDM1α-12F | 5′-GGAGCAGAAATGTTAGGTC-3′ | |
| PRDM1α-12R | 5′-GTGTTGGCTTTAACTACGG-3′ | |
| Exon 7 | 413 | |
| PRDM1α-13F | 5′-CCGTTGGCAACTCTTAATC-3′ | |
| PRDM1α-13R | 5′-TGTCATCCTCCACGTCCTC-3′ | |
| Exon 7 | 494 | |
| PRDM1α-14F | 5′-CACAAGAACTACATCCATC-3′ | |
| PRDM1α-14R | 5′-CAGAGCTGGGATTATGTAC-3′ | |
| PRDM1β | ||
| Intron 3–exon 4 | 264 | |
| PRDM1β-F | 5′-TGGTGGGTTAATCGGTTTGAG-3′ | |
| PRDM1β-R | 5′-GGGATGGGCTTAATGGTGTAG-3′ | |
| IRF4 | ||
| Exon 2–exon 3 | 114 | |
| IRF4-F | 5′-AGAACGAGGAGAAGAGCATC-3′ | |
| IRF4-R | 5′-CCTTTAAACAGTGCCCAAG-3′ | |
| GAPDH | ||
| Exon 1–exon 2 | 255 | |
| GAPDH-F | 5′-GAAGGTGAAGGTCGGAGTC-3′ | |
| GAPDH-R | 5′-GAAGATGGTGATGGGATTTC-3′ | |