Table 3

Presentation of WT1(286-293) by peptide-pulsed B-LCLs with partially matching HLA type

No.Matching alleleNonmatching alleles
Original donor: A1/A24/B8/B40/Cw03/Cw07* 
B-cell-lines not presenting the epitope (IFNγ+ T cells <5%) 
A1 A30/B44/B57/Cw5/Cw6 
A1 A11/B53/B57/Cw4/Cw6 
A24 A32/B35/B44/Cw4/Cw4 
A24/Cw3 A68/B35/B44/Cw4 
A24/Cw3 A3/B15/B35/Cw4 
— A30/A32/B27/B50/Cw1/Cw4 
A24/B40 A32/B35/Cw2/Cw4 
B40/Cw7/Cw7* A203/A20/B38 
B-cell-lines presenting the epitope (IFNγ+ T cells >5%) 
A1/B8/Cw7/Cw7* A2/B18 
10 A1/B8/Cw7/Cw7 A29/A44 
11 A1/B8/Cw7/Cw7 A2/B7 
12 A1/B8/Cw7/Cw7 A3/B49 
13 A1/B8/Cw7/Cw7* A3/B49 
14 A1/B8/Cw3/Cw7 A2/B55 
15 A24/B40/Cw1/ Cw7* A32/B35 
16 B40/Cw3/Cw7 A3/A3/B39 
17 B40/Cw7/Cw7 A203/A20/B38 
18 Cw7/Cw7* A2/A3/B7/B18 
19 Cw72 A3/A3/B7/B41/Cw17 
20 Cw7* A2/A3/B18/B44/Cw12 
21 Cw7 A3/A29/B7/B44/Cw16 
22 Cw7/Cw7 A2/A2/B7/B7 
23 Cw7* A2/A23/B27/B49/C1 
No.Matching alleleNonmatching alleles
Original donor: A1/A24/B8/B40/Cw03/Cw07* 
B-cell-lines not presenting the epitope (IFNγ+ T cells <5%) 
A1 A30/B44/B57/Cw5/Cw6 
A1 A11/B53/B57/Cw4/Cw6 
A24 A32/B35/B44/Cw4/Cw4 
A24/Cw3 A68/B35/B44/Cw4 
A24/Cw3 A3/B15/B35/Cw4 
— A30/A32/B27/B50/Cw1/Cw4 
A24/B40 A32/B35/Cw2/Cw4 
B40/Cw7/Cw7* A203/A20/B38 
B-cell-lines presenting the epitope (IFNγ+ T cells >5%) 
A1/B8/Cw7/Cw7* A2/B18 
10 A1/B8/Cw7/Cw7 A29/A44 
11 A1/B8/Cw7/Cw7 A2/B7 
12 A1/B8/Cw7/Cw7 A3/B49 
13 A1/B8/Cw7/Cw7* A3/B49 
14 A1/B8/Cw3/Cw7 A2/B55 
15 A24/B40/Cw1/ Cw7* A32/B35 
16 B40/Cw3/Cw7 A3/A3/B39 
17 B40/Cw7/Cw7 A203/A20/B38 
18 Cw7/Cw7* A2/A3/B7/B18 
19 Cw72 A3/A3/B7/B41/Cw17 
20 Cw7* A2/A3/B18/B44/Cw12 
21 Cw7 A3/A29/B7/B44/Cw16 
22 Cw7/Cw7 A2/A2/B7/B7 
23 Cw7* A2/A23/B27/B49/C1 

T cells specific for WT-1(286-293) were tested for recogniton of peptide-pulsed B-LCLs with partially matching HLA-type and subsequent INFγ production (CFC).

*

HLA-Cw0701.

HLA-Cw0702.

Presentation only at 25 μg/mL peptide.

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