CD8+ T cells, selected based on their expression of CD137, expand after in vitro culture
| Exp. no. . | Epitope . | % multimer+ before selection . | Total no. of selected cells, ×105 . | % multimer+ cells 1 wk after selection . | Absolute no. of cells after 1 wk, ×106 . | Absolute no. of antigen-specific cells,* ×106 . | Expansion factor, x-fold . |
|---|---|---|---|---|---|---|---|
| 1 | HCV(1406-1415) | 0.39 | 1.1 | 27 | 3.8 | 1.0 | 9.3 |
| 2 | HCV(1406-1415) | 1.6 | 6.0 | 61 | 8.1 | 4.9 | 8.2 |
| 3 | WT1(126-134) | 2.4 | 2.2 | 49 | 2.3 | 1.1 | 5.1 |
| 4 | MelanA(26-35L) | 25 | 4.5 | 72 | 4.5 | 3.2 | 7.2 |
| 5 | MelanA(26-35L) | 8 | 1.4 | 87 | 2.2 | 1.9 | 13.7 |
| Exp. no. . | Epitope . | % multimer+ before selection . | Total no. of selected cells, ×105 . | % multimer+ cells 1 wk after selection . | Absolute no. of cells after 1 wk, ×106 . | Absolute no. of antigen-specific cells,* ×106 . | Expansion factor, x-fold . |
|---|---|---|---|---|---|---|---|
| 1 | HCV(1406-1415) | 0.39 | 1.1 | 27 | 3.8 | 1.0 | 9.3 |
| 2 | HCV(1406-1415) | 1.6 | 6.0 | 61 | 8.1 | 4.9 | 8.2 |
| 3 | WT1(126-134) | 2.4 | 2.2 | 49 | 2.3 | 1.1 | 5.1 |
| 4 | MelanA(26-35L) | 25 | 4.5 | 72 | 4.5 | 3.2 | 7.2 |
| 5 | MelanA(26-35L) | 8 | 1.4 | 87 | 2.2 | 1.9 | 13.7 |
The absolute number of antigen-specific cells was calculated by (the absolute number of cells × percentage of MHC multimer+ cells)/100.