In vitro cytokine-stimulated cell expansion from sorted KLS cells
| Cytokine cocktail . | WT KLS, fold expansion . | Gab2−/− KLS, fold expansion . | WT expansion/Gab2−/− expansion . |
|---|---|---|---|
| IL-36S | 831 ± 156 | 355 ± 190* | 2.3 |
| IL-3SFT | 675 ± 142 | 279 ± 122* | 2.4 |
| IL-3S | 506 ± 169 | 168 ± 110 | 3.0 |
| IL-3F | 150 ± 104 | 25 ± 19 | 6.0 |
| IL-3T | 50 ± 35 | 16 ± 14 | 3.1 |
| SFT | 157 ± 37 | 14 ± 4* | 11.1 |
| Cytokine cocktail . | WT KLS, fold expansion . | Gab2−/− KLS, fold expansion . | WT expansion/Gab2−/− expansion . |
|---|---|---|---|
| IL-36S | 831 ± 156 | 355 ± 190* | 2.3 |
| IL-3SFT | 675 ± 142 | 279 ± 122* | 2.4 |
| IL-3S | 506 ± 169 | 168 ± 110 | 3.0 |
| IL-3F | 150 ± 104 | 25 ± 19 | 6.0 |
| IL-3T | 50 ± 35 | 16 ± 14 | 3.1 |
| SFT | 157 ± 37 | 14 ± 4* | 11.1 |
IL-3 indicates murine IL-3 (20 ng/mL); 6, human IL-6 (50 ng/mL); S, murine stem cell factor (50 ng/mL); F, murine flt-3 ligand (50 ng/mL); and T, murine thrombopoietin (50 ng/mL). KLS cells were sorted by FACS and plated in 96-well plates in the presence of various cytokine cocktails. After 6 days in culture, the total nucleated cell count was determined using a hemacytometer. The fold cell expansion was calculated based on the starting number of cells seeded per well. The results are the averages plus or minus standard deviation for 3 experiments.
*P < .05 relative to wild-type KLS (WT KLS).