Effect of coagulation proteases and PAR peptide agonists on Golgi TF trafficking
| Proteases/peptides . | Percent of cells with Golgi TF pool, mean ± SD . | Colocalization of TF and golgin-97, correlation coefficient, mean ± SD . | TF expression at cell surface, pixel value by image analysis, mean ± SD . |
|---|---|---|---|
| Untreated control | 83.8 ± 4.50 | 0.85 ± 0.017 | 15.9 ± 1.41 |
| FVIIa | 23.3 ± 6.12* | 0.48 ± 0.053* | 25.1 ± 1.15* |
| FFR-FVIIa | 82.9 ± 3.83 | 0.84 ± 0.022 | 16.5 ± 1.84 |
| Thrombin | 26.0 ± 2.90* | 0.42 ± 0.046* | 21.6 ± 3.05* |
| Trypsin | 21.0 ± 5.60* | 0.48 ± 0.036* | 23.5 ± 2.88* |
| FXa | 54.0 ± 8.48* | 0.62 ± 0.063* | 19.1 ± 3.48* |
| PAR1 AP | 22.2 ± 1.16* | 0.44 ± 0.051* | 22.3 ± 2.35* |
| PAR2 AP | 25.4 ± 1.56* | 0.47 ± 0.027* | 22.7 ± 2.59* |
| PAR3 AP | 79.2 ± 3.22 | 0.78 ± 0.085 | 16.9 ± 1.04 |
| PAR4 AP | 80.5 ±5.15 | 0.85 ± 0.050 | 15.8 ± 1.53 |
| Proteases/peptides . | Percent of cells with Golgi TF pool, mean ± SD . | Colocalization of TF and golgin-97, correlation coefficient, mean ± SD . | TF expression at cell surface, pixel value by image analysis, mean ± SD . |
|---|---|---|---|
| Untreated control | 83.8 ± 4.50 | 0.85 ± 0.017 | 15.9 ± 1.41 |
| FVIIa | 23.3 ± 6.12* | 0.48 ± 0.053* | 25.1 ± 1.15* |
| FFR-FVIIa | 82.9 ± 3.83 | 0.84 ± 0.022 | 16.5 ± 1.84 |
| Thrombin | 26.0 ± 2.90* | 0.42 ± 0.046* | 21.6 ± 3.05* |
| Trypsin | 21.0 ± 5.60* | 0.48 ± 0.036* | 23.5 ± 2.88* |
| FXa | 54.0 ± 8.48* | 0.62 ± 0.063* | 19.1 ± 3.48* |
| PAR1 AP | 22.2 ± 1.16* | 0.44 ± 0.051* | 22.3 ± 2.35* |
| PAR2 AP | 25.4 ± 1.56* | 0.47 ± 0.027* | 22.7 ± 2.59* |
| PAR3 AP | 79.2 ± 3.22 | 0.78 ± 0.085 | 16.9 ± 1.04 |
| PAR4 AP | 80.5 ±5.15 | 0.85 ± 0.050 | 15.8 ± 1.53 |
Values are based on 3 to 7 independent experiments.
Significantly differs from the value obtained with untreated control (P < .01).