Relative expression levels of various downstream targets in Akt-active and Akt-inactivate MCL cases from RPPA assay
Akt target . | MCL with p-Akt, mean (σ)* . | MCL without p-Akt, mean (σ)* . | P† . |
|---|---|---|---|
| P-MDM2 (Ser166) | 3.31 (0.29) | 1.17 (0.17) | < .001 |
| P-Bad (Ser136) | 5.0 (0.35) | 1.37 (0.37) | < .001 |
| P-FKHRL-1 (Ser253) | 4.4 (0.27) | 1.90 (0.32) | < .001 |
| P-GSK-3β (Ser9) | 4.57 (0.36) | 3.28 (0.36) | .042 |
| P-mTOR (Ser2448) | 5.03 (0.35) | 2.22 (0.56) | .001 |
| P-p70S6K (Thr389) | 2.61 (0.38) | 1.0 (0.10) | .013 |
| P-S6K (Ser235/236) | 4.09 (0.21) | 1.88 (0.38) | < .001 |
Akt target . | MCL with p-Akt, mean (σ)* . | MCL without p-Akt, mean (σ)* . | P† . |
|---|---|---|---|
| P-MDM2 (Ser166) | 3.31 (0.29) | 1.17 (0.17) | < .001 |
| P-Bad (Ser136) | 5.0 (0.35) | 1.37 (0.37) | < .001 |
| P-FKHRL-1 (Ser253) | 4.4 (0.27) | 1.90 (0.32) | < .001 |
| P-GSK-3β (Ser9) | 4.57 (0.36) | 3.28 (0.36) | .042 |
| P-mTOR (Ser2448) | 5.03 (0.35) | 2.22 (0.56) | .001 |
| P-p70S6K (Thr389) | 2.61 (0.38) | 1.0 (0.10) | .013 |
| P-S6K (Ser235/236) | 4.09 (0.21) | 1.88 (0.38) | < .001 |
The mean expression levels for each target were calculated from 17 typical MCL and 12 blastoid variants
Mean expression levels (log2) with standard deviations calculated, as described in “Reverse-phase protein assays”
Independent 2-tailed Student t test performed for MCL cases with phosphorylated Akt versus MCL without phosphorylated Akt