Effect of depletion of SAA from serum on opsonic activity
. | % GFP+ neutrophils . | . | . | ||
|---|---|---|---|---|---|
. | No depletion . | Anti-SAA depleted . | HIC depleted . | ||
| Donor A | 66 | 37 | 53 | ||
| Donor B | 16 | 11 | 12 | ||
| Donor C | 36 | 52 | 53 | ||
| Mean ± SEM, 8 donors | 43.0 ± 8.1 | 40.1 ± 6.2 | 40.8 ± 7.8 | ||
. | % GFP+ neutrophils . | . | . | ||
|---|---|---|---|---|---|
. | No depletion . | Anti-SAA depleted . | HIC depleted . | ||
| Donor A | 66 | 37 | 53 | ||
| Donor B | 16 | 11 | 12 | ||
| Donor C | 36 | 52 | 53 | ||
| Mean ± SEM, 8 donors | 43.0 ± 8.1 | 40.1 ± 6.2 | 40.8 ± 7.8 | ||
Normal serum or SAA-depleted normal serum was added to E coli–GFP and neutrophils for 30 minutes at 37°C, and phagocytosis was determined by FACS analysis of cells in the presence or absence of cytochalasin D. Phagocytosis was expressed as the difference between percentage of GFP+ cells with or without cytochalasin D