EGFP-expressing c-kit+/lin– cells from the bone marrow of induced SCL-tTA-2S/EGFP-lacZ mice contain early and late CAFC activity
. | Experiment 1, CAFCs per 104 cells . | . | Experiment 2, CAFCs per 104 cells . | . | ||
|---|---|---|---|---|---|---|
| Tested cell population . | Day 14 . | Day 35 . | Day 14 . | Day 35 . | ||
| MNCs | 1.2 (0.8-1.7) | 0.25 (0.2-0.3) | 1 (0.6-1.3) | 0.2 (0.1-0.3) | ||
| lin c-kit+ EGFP+ | 72 (41-102) | 15 (4-26) | 18 (9-27) | 6 (2-11) | ||
| lin– c-kit+ EGFP– | 38 (24-52) | 5 (3-7) | 35 (22-47) | 10 (5-14) | ||
. | Experiment 1, CAFCs per 104 cells . | . | Experiment 2, CAFCs per 104 cells . | . | ||
|---|---|---|---|---|---|---|
| Tested cell population . | Day 14 . | Day 35 . | Day 14 . | Day 35 . | ||
| MNCs | 1.2 (0.8-1.7) | 0.25 (0.2-0.3) | 1 (0.6-1.3) | 0.2 (0.1-0.3) | ||
| lin c-kit+ EGFP+ | 72 (41-102) | 15 (4-26) | 18 (9-27) | 6 (2-11) | ||
| lin– c-kit+ EGFP– | 38 (24-52) | 5 (3-7) | 35 (22-47) | 10 (5-14) | ||
Bone marrow cells were isolated and cultured on OP-9 cells for limiting dilution analysis of CAFC activity as described in “Materials and methods.” Mean CAFC frequencies scored at day 14 and day 35 are shown for 2 independent experiments using, in total, 4 different mice. Numbers in parentheses indicate the range of the 95% confidence limit
MNCs indicates mononuclear cells