C3bR and FcγR expression in differentiated PLB and PLB-D cells
Type of receptor . | PLB, median . | PLB-D, median . |
|---|---|---|
| FcγRI | ||
| Undifferentiated | 8.7 ± 1.5 | 9.1 ± 2.5 |
| Vitamin D | 8.1 ± 1.8 | 8.5 ± 1.9 |
| FcγRIIA | ||
| Undifferentiated | 11.5 ± 2.5 | 9.4 ± 2.1 |
| Vitamin D | 31.1 ± 4.2 | 8.5 ± 2.1 |
| FcγRIII | ||
| Undifferentiated | 26.4 ± 2.1 | 24.9 ± 5.4 |
| Vitamin D | 27.5 ± 3.8 | 26.3 ± 3.1 |
| CR1 | ||
| Undifferentiated | 23.6 ± 3.9 | 18.9 ± 0.9 |
| Vitamin D | 60.8 ± 6.5 | 52.5 ± 6.9 |
| CR3 | ||
| Undifferentiated | 8.9 ± 4.5 | 9.2 ± 2.8 |
| Vitamin D | 54.4 ± 6.3 | 55.4 ± 7.2 |
Type of receptor . | PLB, median . | PLB-D, median . |
|---|---|---|
| FcγRI | ||
| Undifferentiated | 8.7 ± 1.5 | 9.1 ± 2.5 |
| Vitamin D | 8.1 ± 1.8 | 8.5 ± 1.9 |
| FcγRIIA | ||
| Undifferentiated | 11.5 ± 2.5 | 9.4 ± 2.1 |
| Vitamin D | 31.1 ± 4.2 | 8.5 ± 2.1 |
| FcγRIII | ||
| Undifferentiated | 26.4 ± 2.1 | 24.9 ± 5.4 |
| Vitamin D | 27.5 ± 3.8 | 26.3 ± 3.1 |
| CR1 | ||
| Undifferentiated | 23.6 ± 3.9 | 18.9 ± 0.9 |
| Vitamin D | 60.8 ± 6.5 | 52.5 ± 6.9 |
| CR3 | ||
| Undifferentiated | 8.9 ± 4.5 | 9.2 ± 2.8 |
| Vitamin D | 54.4 ± 6.3 | 55.4 ± 7.2 |
Cells were differentiated for 4 days with 1,25(OH)2D3. Results are mean ± SEM of 3 independent experiments. The mean ± SEM of negative controls for FcγRIIA in undifferentiated PLB and PLB-D cells was 5.2 ± 0.6 and 5.8 ± 0.7, respectively, and in differentiated cells it was 4.6 ± 0.6 and 4.7 ± 0.43, respectively. The levels of FcγRIIA were not significantly different in undifferentiated PLB and PLB-D cells from the negative controls (P = .06 and P = .17, respectively) and in differentiated PLB-D cells (P = .15). The mean ± SEM of negative controls for all other receptors in undifferentiated PLB and PLB-D cells was 4.7 ± 0.3 and 4.7 ± 0.4, respectively, and in differentiated cells it was 4.7 ± 0.6 and 4.7 ± 0.5, respectively. The levels of FcγRI or CR3 were not significantly different in undifferentiated PLB and PLB-D cells from their negative controls (P > .19)