Real time polymerase chain reaction primers
Genes . | Primer sequences . | Amplicon size, bp . |
|---|---|---|
| KLF1 | 5′-cgg aca cac agg atg act tc-3′ forward | 115 |
| 5′-ggc tgg tcc tca gac ttc ac-3′ reverse | ||
| KLF2 | 5′-cat ctg aag gcg cat ctg-3′ forward | 120 |
| 5′-cgt gtg ctt tcg gta gtg g-3′ reverse | ||
| GATA1 | 5′-ggg atc aca ctg agc ttg c-3′ forward | 176 |
| 5′-acc cct gat tct ggt gtg g-3′ reverse | ||
| GATA2 | 5′-aag gct cgt tcc tgt tca ga-3′ forward | 102 |
| 5′-ggc att gca cag gta gtg g-3′ reverse | ||
| MYB | 5′-gtc aca aat tga ctg tta caa cac cat-3′ forward | 212 |
| 5′-ttc tac tag atg aga ggg tgt ctg agg-3′ reverse | ||
| β-actin | 5′-cca acc gcg aga aga tga-3′ forward | 97 |
| 5′-cca gag gcg tac agg gat ag-3′ reverse |
Genes . | Primer sequences . | Amplicon size, bp . |
|---|---|---|
| KLF1 | 5′-cgg aca cac agg atg act tc-3′ forward | 115 |
| 5′-ggc tgg tcc tca gac ttc ac-3′ reverse | ||
| KLF2 | 5′-cat ctg aag gcg cat ctg-3′ forward | 120 |
| 5′-cgt gtg ctt tcg gta gtg g-3′ reverse | ||
| GATA1 | 5′-ggg atc aca ctg agc ttg c-3′ forward | 176 |
| 5′-acc cct gat tct ggt gtg g-3′ reverse | ||
| GATA2 | 5′-aag gct cgt tcc tgt tca ga-3′ forward | 102 |
| 5′-ggc att gca cag gta gtg g-3′ reverse | ||
| MYB | 5′-gtc aca aat tga ctg tta caa cac cat-3′ forward | 212 |
| 5′-ttc tac tag atg aga ggg tgt ctg agg-3′ reverse | ||
| β-actin | 5′-cca acc gcg aga aga tga-3′ forward | 97 |
| 5′-cca gag gcg tac agg gat ag-3′ reverse |