Comparison of the percentage reductions in proplatelet formation and adhesion on fibrinogen
. | Reduction in adherent megakaryocytes, % . | Reduction of megakaryocytes with proplatelets, % . | P . |
|---|---|---|---|
| Lotrafiban | 63.2 ± 11.3 | 99.1 ± 1.5 | .034 |
| Anti-αIIb | 83.3 ± 1.63 | 99.4 ± 0.64 | < .001 |
. | Reduction in adherent megakaryocytes, % . | Reduction of megakaryocytes with proplatelets, % . | P . |
|---|---|---|---|
| Lotrafiban | 63.2 ± 11.3 | 99.1 ± 1.5 | .034 |
| Anti-αIIb | 83.3 ± 1.63 | 99.4 ± 0.64 | < .001 |
Bone marrow aspirates from TPO-treated mice were treated with lotrafiban or anti-αIIb antibody and plated on 13-mm coverslips coated with fibrinogen and allowed to adhere for 3 to 4 hours. The total number of megakaryocytes and megakaryocytes bearing proplatelets were counted for the entire coverslip, and converted to a percentage of cells compared with control treated cells and are displayed ± SEM; n = 3