Macrophage protection by ACM from different cell lines and stimuli
Cell line and stimulus . | Protection, %* . | SD, n ≥ 3 . |
|---|---|---|
| Jurkat | ||
| Staurosporine, 0.5 μg/mL, 3 h | 100 | ± 0 |
| Anti-Fas, 0.05 ng/mL, 5 h | 91.1 | ± 18.6 |
| RKO | ||
| Staurosporine, 0.5 μg/mL, 5 h | 103.3 | ± 23.6 |
| MCF-7 | ||
| Staurosporine, 0.5 μg/mL, 4 h | 91.3 | ± 12.9 |
| HEK293 | ||
| Staurosporine, 0.5 μg/mL, 8 h | 80.5 | ± 19.7 |
| TNF-α/CHX, 50 ng/mL/10 μg/mL, 24 h | 106.7 | ± 27.5 |
Cell line and stimulus . | Protection, %* . | SD, n ≥ 3 . |
|---|---|---|
| Jurkat | ||
| Staurosporine, 0.5 μg/mL, 3 h | 100 | ± 0 |
| Anti-Fas, 0.05 ng/mL, 5 h | 91.1 | ± 18.6 |
| RKO | ||
| Staurosporine, 0.5 μg/mL, 5 h | 103.3 | ± 23.6 |
| MCF-7 | ||
| Staurosporine, 0.5 μg/mL, 4 h | 91.3 | ± 12.9 |
| HEK293 | ||
| Staurosporine, 0.5 μg/mL, 8 h | 80.5 | ± 19.7 |
| TNF-α/CHX, 50 ng/mL/10 μg/mL, 24 h | 106.7 | ± 27.5 |
100% protection is set as the difference in caspase-3 activity (fold of control) between untreated THP-1 macrophages and those treated with ACM (16 hours) from Jurkat cells killed with staurosporine, followed by induction of apoptosis in macrophages with 250 μM etoposide for 8 hours. Caspase-3 activity in whole-cell lysates was quantified as described in “Materials and methods.” Differences in caspase-3 activity between untreated and ACM-treated macrophages were significant (P < .05) for each cell line and stimulus indicated