Long-term repopulation of secondary irradiated recipients with α4 integrin–/– HSCs
. | Primary tissue, % . | . | |
|---|---|---|---|
. | E12.5 AGM region . | E12.5 fetal liver . | |
| Primary recipient PB | 35 | 51 | |
| Primary recipient BM | 11 | 14 | |
| Secondary recipient peripheral blood | |||
| 1.1 × 105 donor BM cells | ND | 3, 4 | |
| 2.2 × 105 donor BM cells | ND | 10 | |
| 5.5 × 105 donor BM cells | ND | 9, 14, 19 | |
| 1.4 × 106 donor BM cells | 14, 14, 14 | ND | |
. | Primary tissue, % . | . | |
|---|---|---|---|
. | E12.5 AGM region . | E12.5 fetal liver . | |
| Primary recipient PB | 35 | 51 | |
| Primary recipient BM | 11 | 14 | |
| Secondary recipient peripheral blood | |||
| 1.1 × 105 donor BM cells | ND | 3, 4 | |
| 2.2 × 105 donor BM cells | ND | 10 | |
| 5.5 × 105 donor BM cells | ND | 9, 14, 19 | |
| 1.4 × 106 donor BM cells | 14, 14, 14 | ND | |
Analysis of the peripheral blood of secondary recipients shows significant contribution of α4 integrin-/- cells 12 weeks after transplantation. Percentages indicate the level of contribution for each primary or secondary recipient, multiple numbers represent each mouse that received the corresponding secondary BM transplant.
PB indicates peripheral blood; BM, bone marrow; and ND, not done.