Statistical analysis of α4 integrin–/– and wild-type donor cell subsets in recipient tissues
. | Wild type, mean ± SEM (no.) . | α4 integrin-/-, mean ± SEM (no.) . | P . |
|---|---|---|---|
| Peripheral blood | |||
| CD3ϵ+ | 21.8 ± 3.5 (6) | 21.0 ± 6.9 (8) | .92 |
| B220+ | 54.8 ± 3.1 (6) | 59.4 ± 5.7 (8) | .50 |
| CD11b+Gr1- | 5.7 ± 1.3 (6) | 7.4 ± 1.9 (7) | .46 |
| CD11b+Gr1+ | 13.1 ± 3.0 (6) | 10.3 ± 2.7 (7) | .50 |
| Bone marrow | |||
| B220+ | 29.0 ± 6.0 (9) | 24.3 ± 2.4 (10) | .48 |
| CD11b+Gr1- | 10.9 ± 1.2 (9) | 9.4 ± 1.1 (8) | .37 |
| CD11b+Gr1+ | 51.6 ± 5.9 (9) | 49.6 ± 6.4 (8) | .74 |
| B220+IgD+ | 9.8 ± 0.5 (5) | 17.0 ± 3.2 (3) | .15 |
| B220+IgD- | 12.2 ± 4.5 (5) | 5.5 ± 1.6 (3) | .22 |
| Spleen | |||
| CD3ϵ+ | 20.6 ± 2.9 (5) | 24.2 ± 3.5 (6) | .46 |
| B220+ | 64.0 ± 2.0 (5) | 63.0 ± 3.9 (6) | .83 |
| CD11b+Gr1- | 5.2 ± 1.4 (5) | 4.1 ± 0.6 (5) | .51 |
| CD11b+Gr1+ | 3.2 ± 0.8 (5) | 1.5 ± 0.5 (5) | .12 |
| CD19+IgM+ | 57.0 ± 5.0 (3) | 55.8 ± 5.3 (4) | .87 |
| CD19+IgM- | 15.3 ± 3.2 (3) | 14.0 ± 2.9 (4) | .77 |
| CD19+AA4.1+ | 8.8 ± 1.0 (6) | 10.3 ± 0.7 (3) | .26 |
| CD19+AA4.1- | 64.8 ± 2.8 (6) | 66.0 ± 2.9 (3) | .78 |
| Thymus | |||
| CD4 SP | 10.7 ± 1.9 (6) | 13.8 ± 1.9 (5) | .28 |
| CD8α SP | 2.8 ± 0.9 (6) | 4.0 ± 0.6 (5) | .32 |
| CD4+CD8α+ | 77.0 ± 7.2 (6) | 67.2 ± 6.8 (5) | .35 |
| Lymph nodes | |||
| CD4 SP | 47.3 ± 3.3 (6) | 40.2 ± 5.4 (5) | .30 |
| CD8α SP | 21.0 ± 1.7 (6) | 18.4 ± 1.9 (5) | .34 |
| Peyer patches | |||
| CD4 SP | 23.9 ± 4.9 (7) | 45.4 ± 7.4 (8) | .03 |
| CD8α SP | 7.7 ± 1.6 (7) | 7.8 ± 1.4 (8) | .90 |
| B220+ | 69.7 ± 5.5 (7) | 25.5 ± 5.3 (8) | <.001 |
| B220+IgM+ | 40.0 (1) | 6.4 ± 1.5 (5) | NA |
| B220+IgD+ | 52.7 ± 10.0 (3) | ND | NA |
| B220+CD5+ | 15.0 ± 0.7 (4) | 13.1 ± 1.8 (5) | .40 |
| Peritoneal cavity | |||
| CD11b+ | 80.8 ± 4.4 (5) | 44.1 ± 8.4 (8) | <.01 |
| CD3ϵ+ | 19.2 ± 5.8 (5) | 21.1 ± 4.1 (7) | .80 |
| B220+ | 47.3 ± 2.7 (8) | 33.7 ± 9.0 (7) | .20 |
| B220+CD5+ | 12.5 ± 1.4 (4) | 3.9 ± 1.7 (5) | <.01 |
. | Wild type, mean ± SEM (no.) . | α4 integrin-/-, mean ± SEM (no.) . | P . |
|---|---|---|---|
| Peripheral blood | |||
| CD3ϵ+ | 21.8 ± 3.5 (6) | 21.0 ± 6.9 (8) | .92 |
| B220+ | 54.8 ± 3.1 (6) | 59.4 ± 5.7 (8) | .50 |
| CD11b+Gr1- | 5.7 ± 1.3 (6) | 7.4 ± 1.9 (7) | .46 |
| CD11b+Gr1+ | 13.1 ± 3.0 (6) | 10.3 ± 2.7 (7) | .50 |
| Bone marrow | |||
| B220+ | 29.0 ± 6.0 (9) | 24.3 ± 2.4 (10) | .48 |
| CD11b+Gr1- | 10.9 ± 1.2 (9) | 9.4 ± 1.1 (8) | .37 |
| CD11b+Gr1+ | 51.6 ± 5.9 (9) | 49.6 ± 6.4 (8) | .74 |
| B220+IgD+ | 9.8 ± 0.5 (5) | 17.0 ± 3.2 (3) | .15 |
| B220+IgD- | 12.2 ± 4.5 (5) | 5.5 ± 1.6 (3) | .22 |
| Spleen | |||
| CD3ϵ+ | 20.6 ± 2.9 (5) | 24.2 ± 3.5 (6) | .46 |
| B220+ | 64.0 ± 2.0 (5) | 63.0 ± 3.9 (6) | .83 |
| CD11b+Gr1- | 5.2 ± 1.4 (5) | 4.1 ± 0.6 (5) | .51 |
| CD11b+Gr1+ | 3.2 ± 0.8 (5) | 1.5 ± 0.5 (5) | .12 |
| CD19+IgM+ | 57.0 ± 5.0 (3) | 55.8 ± 5.3 (4) | .87 |
| CD19+IgM- | 15.3 ± 3.2 (3) | 14.0 ± 2.9 (4) | .77 |
| CD19+AA4.1+ | 8.8 ± 1.0 (6) | 10.3 ± 0.7 (3) | .26 |
| CD19+AA4.1- | 64.8 ± 2.8 (6) | 66.0 ± 2.9 (3) | .78 |
| Thymus | |||
| CD4 SP | 10.7 ± 1.9 (6) | 13.8 ± 1.9 (5) | .28 |
| CD8α SP | 2.8 ± 0.9 (6) | 4.0 ± 0.6 (5) | .32 |
| CD4+CD8α+ | 77.0 ± 7.2 (6) | 67.2 ± 6.8 (5) | .35 |
| Lymph nodes | |||
| CD4 SP | 47.3 ± 3.3 (6) | 40.2 ± 5.4 (5) | .30 |
| CD8α SP | 21.0 ± 1.7 (6) | 18.4 ± 1.9 (5) | .34 |
| Peyer patches | |||
| CD4 SP | 23.9 ± 4.9 (7) | 45.4 ± 7.4 (8) | .03 |
| CD8α SP | 7.7 ± 1.6 (7) | 7.8 ± 1.4 (8) | .90 |
| B220+ | 69.7 ± 5.5 (7) | 25.5 ± 5.3 (8) | <.001 |
| B220+IgM+ | 40.0 (1) | 6.4 ± 1.5 (5) | NA |
| B220+IgD+ | 52.7 ± 10.0 (3) | ND | NA |
| B220+CD5+ | 15.0 ± 0.7 (4) | 13.1 ± 1.8 (5) | .40 |
| Peritoneal cavity | |||
| CD11b+ | 80.8 ± 4.4 (5) | 44.1 ± 8.4 (8) | <.01 |
| CD3ϵ+ | 19.2 ± 5.8 (5) | 21.1 ± 4.1 (7) | .80 |
| B220+ | 47.3 ± 2.7 (8) | 33.7 ± 9.0 (7) | .20 |
| B220+CD5+ | 12.5 ± 1.4 (4) | 3.9 ± 1.7 (5) | <.01 |
ND indicates not determined; NA, not applicable; and SP, single positive.