Coexistence of donor embryonic cells with competitor BM cells
. | Wild-type donor . | . | . | . | α4 integrin-/- donor . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | Mouse 1, AGM . | Mouse 2, YS . | Mouse 3, PB . | Mouse 4, FL . | Mouse 1, AGM . | Mouse 2, YS . | Mouse 3, PB . | Mouse 4, FL . | ||||||
| Embryonic donor, % | 54 | 33 | 16 | 42 | 34 | 35 | 60 | 47 | ||||||
| Competitive bone marrow, % | 14 | 9 | 35 | 9 | 44 | 13 | 10 | 27 | ||||||
. | Wild-type donor . | . | . | . | α4 integrin-/- donor . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | Mouse 1, AGM . | Mouse 2, YS . | Mouse 3, PB . | Mouse 4, FL . | Mouse 1, AGM . | Mouse 2, YS . | Mouse 3, PB . | Mouse 4, FL . | ||||||
| Embryonic donor, % | 54 | 33 | 16 | 42 | 34 | 35 | 60 | 47 | ||||||
| Competitive bone marrow, % | 14 | 9 | 35 | 9 | 44 | 13 | 10 | 27 | ||||||
In conjunction with Figures 5A and 6, this table illustrates clearly that there is no defect in homing or multilineage differentiation of α4 integrin-/- embryonic HSCs in the presence of substantial numbers of competitor BM cells (as compared to similar embryonic wild-type HSCs contransplanted with competitor BM cells).
The embryonic donor organ is given for each recipient mouse. Analysis of peripheral blood for donor and competitive BM percentages was performed 12 weeks after transplantation.
AGM indicates aorta-gonads-mesonephros region; YS, yolk sac; PB, peripheral blood; and FL, fetal liver.