Donor-derived cells reisolated from different lymphoid organs of Balb/c recipients at day 7 after transplantation in absolute cell numbers
. | Donor TCONV cells, × 105 . | . | Donor Treg cells, × 105 . | |
|---|---|---|---|---|
| Lymphoid organ, group . | CD4+/Thy-1.2+ . | CD8+/Thy-1.2+ . | CD4+/FOXP3+/Thy-1.1+ . | |
| Spleen | ||||
| TCONV | 7.3 ± 0.8 | 4.2 ± 0.9 | — | |
| TCONV + Treg | 2.4 ± 0.6 | 1.5 ± 0.3 | 1.5 ± 0.3 | |
| TCONV + Treg + CSA | 4.8 ± 1.2 | 2.8 ± 0.9 | 0.4 ± 0.2 | |
| TCONV + Treg + RAPA | 2.7 ± 0.9 | 1.9 ± 0.4 | 1.6 ± 0.5 | |
| TCONV + Treg + MMF | 3.1 ± 0.4 | 1.7 ± 0.5 | 1.2 ± 0.1 | |
| cLN | ||||
| TCONV | 2.6 ± 0.4 | 1.6 ± 0.4 | — | |
| TCONV + Treg | 1.0 ± 0.3 | 0.8 ± 0.1 | 0.4 ± 0.1 | |
| TCONV + Treg + CSA | 2.1 ± 0.4 | 1.0 ± 0.3 | 0.1 ± 0.1 | |
| TCONV + Treg + RAPA | 0.9 ± 0.1 | 0.6 ± 0.3 | 0.5 ± 0.1 | |
| TCONV + Treg + MMF | 1.0 ± 0.3 | 0.6 ± 0.5 | 0.3 ± 0.3 | |
| mLN | ||||
| TCONV | 3.9 ± 0.9 | 1.6 ± 0.4 | — | |
| TCONV + Treg | 1.4 ± 0.6 | 0.5 ± 0.2 | 0.9 ± 0.2 | |
| TCONV + Treg + CSA | 2.7 ± 0.2 | 1.1 ± 0.3 | 0.3 ± 0.1 | |
| TCONV + Treg + RAPA | 1.7 ± 0.3 | 0.4 ± 0.1 | 0.8 ± 0.2 | |
| TCONV + Treg + MMF | 1.4 ± 0.6 | 0.6 ± 0.1 | 0.7 ± 0.1 | |
. | Donor TCONV cells, × 105 . | . | Donor Treg cells, × 105 . | |
|---|---|---|---|---|
| Lymphoid organ, group . | CD4+/Thy-1.2+ . | CD8+/Thy-1.2+ . | CD4+/FOXP3+/Thy-1.1+ . | |
| Spleen | ||||
| TCONV | 7.3 ± 0.8 | 4.2 ± 0.9 | — | |
| TCONV + Treg | 2.4 ± 0.6 | 1.5 ± 0.3 | 1.5 ± 0.3 | |
| TCONV + Treg + CSA | 4.8 ± 1.2 | 2.8 ± 0.9 | 0.4 ± 0.2 | |
| TCONV + Treg + RAPA | 2.7 ± 0.9 | 1.9 ± 0.4 | 1.6 ± 0.5 | |
| TCONV + Treg + MMF | 3.1 ± 0.4 | 1.7 ± 0.5 | 1.2 ± 0.1 | |
| cLN | ||||
| TCONV | 2.6 ± 0.4 | 1.6 ± 0.4 | — | |
| TCONV + Treg | 1.0 ± 0.3 | 0.8 ± 0.1 | 0.4 ± 0.1 | |
| TCONV + Treg + CSA | 2.1 ± 0.4 | 1.0 ± 0.3 | 0.1 ± 0.1 | |
| TCONV + Treg + RAPA | 0.9 ± 0.1 | 0.6 ± 0.3 | 0.5 ± 0.1 | |
| TCONV + Treg + MMF | 1.0 ± 0.3 | 0.6 ± 0.5 | 0.3 ± 0.3 | |
| mLN | ||||
| TCONV | 3.9 ± 0.9 | 1.6 ± 0.4 | — | |
| TCONV + Treg | 1.4 ± 0.6 | 0.5 ± 0.2 | 0.9 ± 0.2 | |
| TCONV + Treg + CSA | 2.7 ± 0.2 | 1.1 ± 0.3 | 0.3 ± 0.1 | |
| TCONV + Treg + RAPA | 1.7 ± 0.3 | 0.4 ± 0.1 | 0.8 ± 0.2 | |
| TCONV + Treg + MMF | 1.4 ± 0.6 | 0.6 ± 0.1 | 0.7 ± 0.1 | |
On day 7 after BMT, lymphoid organs were removed and total cells per organ were counted from a single-cell suspension. Subsequent analysis of the resulting cell suspensions by FACS provided the percentages based on which the absolute cell numbers were calculated. Data are derived from 3 independent experiments.
— indicates below detection.