Detection of KSHV genomic DNA in clonogenic colonies derived from infected CD34+ HPCs
. | KSHV+ colonies/total colonies analyzed . | . | . | ||
|---|---|---|---|---|---|
. | CFU-GM . | BFU-E . | HPP . | ||
| KSHV | 17% (15 of 90)* | 4% (1 of 23)† | 0% (0 of 20) | ||
| UV-KSHV | 1% (1 of 67)‡ | 0% (0 of 12) | 0% (0 of 17) | ||
| Mock | 0% (0 of 25) | 0% (0 of 7) | 0% (0 of 8) | ||
. | KSHV+ colonies/total colonies analyzed . | . | . | ||
|---|---|---|---|---|---|
. | CFU-GM . | BFU-E . | HPP . | ||
| KSHV | 17% (15 of 90)* | 4% (1 of 23)† | 0% (0 of 20) | ||
| UV-KSHV | 1% (1 of 67)‡ | 0% (0 of 12) | 0% (0 of 17) | ||
| Mock | 0% (0 of 25) | 0% (0 of 7) | 0% (0 of 8) | ||
CD34+ HPCs were exposed to KSHV or UV-irradiated KSHV (10 μW/cm2 for 1 hour). Cells were washed 3 times with PBS and plated into MethoCult H4433. Colonies were visually scored, randomly isolated, and DNA was analyzed by real-time PCR analysis at 12 to 15 days after plating. Experiments were performed 3 times. Colonies with less than 1.0 calculated viral copy per cell were scored as negative for KSHV infection. Colonies demonstrating less than 2 human β-globin copies were excluded from analysis.
17 ± 5.6 mean KSHV genomic copies per human cell in positive CFU-GM colonies.
3.3 KSHV genomic copies per human cell in positive BFU-E colony.
2.7 KSHV genomic copies per human cell in positive CFU-GM colony.