Conditions of amplification (PCR) and identification of polymorphisms
Genes . | Primers, 5′-3′ . | Annealing temperature, °C (cycles) . | References . |
|---|---|---|---|
| MTHFR C677T | F, TGAAGGAGAAGGTGTCTGCGGGA | 66 (40) | Matsuo et al15 |
| R, AGGACGGTGCGGTGAGAGTG | |||
| MTHFR A1298C | F, CTTTGGGGAGCGAAGGACTACA | 60 (40) | Matsuo et al15 |
| R, CACTTTGTGACCATTCCGGTTTG | |||
| MTR A2756G | F, TGTTCCAGACAGTTAGATGAAAATC | 60 (40) | Matsuo et al15 |
| R, GATCCAAAGCCTTTTACACTCCTC | |||
| SHMT C1240T | F, GTGTGGGGTGACTTCATTTGTG | Touchdown 60-52 (40) | Skibola et al12 |
| R, GGAGCAGCTCATCCATCTCTC | |||
| TYMS tandem repeats | F, CGTGGCTCCTGCGTTTCC | 61 (40) | Hishida et al16 |
| R, GAGCCGGCCACAGGCAT |
Genes . | Primers, 5′-3′ . | Annealing temperature, °C (cycles) . | References . |
|---|---|---|---|
| MTHFR C677T | F, TGAAGGAGAAGGTGTCTGCGGGA | 66 (40) | Matsuo et al15 |
| R, AGGACGGTGCGGTGAGAGTG | |||
| MTHFR A1298C | F, CTTTGGGGAGCGAAGGACTACA | 60 (40) | Matsuo et al15 |
| R, CACTTTGTGACCATTCCGGTTTG | |||
| MTR A2756G | F, TGTTCCAGACAGTTAGATGAAAATC | 60 (40) | Matsuo et al15 |
| R, GATCCAAAGCCTTTTACACTCCTC | |||
| SHMT C1240T | F, GTGTGGGGTGACTTCATTTGTG | Touchdown 60-52 (40) | Skibola et al12 |
| R, GGAGCAGCTCATCCATCTCTC | |||
| TYMS tandem repeats | F, CGTGGCTCCTGCGTTTCC | 61 (40) | Hishida et al16 |
| R, GAGCCGGCCACAGGCAT |
F indicates forward; R, reverse.