Macrophage colonies are increased in response to M-CSF in clonal cultures of VavP-MYC bone marrow cells
. | Mean no. colonies, ± SD . | . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Stimulus . | Blast . | Granulocyte . | Granulocyte-macrophage . | Macrophage . | Eosinophil . | Megakaryocyte . | |||||
| Wild-type, 2 wk; n = 3 | |||||||||||
| GM-CSF | 0 | 15 ± 4 | 7 ± 4 | 17 ± 4 | 2 ± 1 | 0 | |||||
| M-CSF | 0 | 0.7 ± 0.6 | 3 ± 1 | 50 ± 17* | 0 | 0 | |||||
| IL-3 | 8 ± 2 | 12 ± 4 | 8 ± 3 | 11 ± 12 | 2 ± 2 | 5 ± 2 | |||||
| SCF + IL-3 + EPO | 8 ± 3 | 18 ± 6 | 12 ± 11 | 11 ± 12 | 2 ± 2 | 24 ± 5 | |||||
| MYC17, 2 wk; n = 3 | |||||||||||
| GM-CSF | 0 | 10 ± 2 | 4 ± 0 | 19 ± 8 | 2 ± 1 | 0 | |||||
| M-CSF | 0 | 0 | 2 ± 2 | 158 ± 48* | 0 | 0 | |||||
| IL-3 | 5 ± 2 | 9 ± 3 | 3 ± 3 | 13 ± 6 | 3 ± 3 | 6 ± 2 | |||||
| SCF + IL-3 + EPO | 8 ± 3 | 7 ± 3 | 3 ± 2 | 13 ± 15 | 0.7 ± 1.2 | 11 ± 4 | |||||
| Wild-type, 8 wk; n = 4 | |||||||||||
| GM-CSF | 0 | 16 ± 5 | 5 ± 2 | 31 ± 3 | 4 ± 2 | 0 | |||||
| M-CSF | 0 | 3 ± 1 | 6 ± 4 | 49 ± 8* | 0 | 0 | |||||
| IL-3 | 7 ± 4 | 19 ± 6 | 11 ± 3 | 15 ± 3 | 2 ± 1 | 7 ± 4 | |||||
| SCF + IL-3 + EPO | 11 ± 6 | 21 ± 2 | 13 ± 3 | 17 ± 3 | 2 ± 1 | 28 ± 4 | |||||
| MYC10, 8 wk; n = 4 | |||||||||||
| GM-CSF | 0 | 19 ± 6 | 5 ± 2 | 29 ± 15 | 3 ± 3 | 0 | |||||
| M-CSF | 0 | 3 ± 1 | 2 ± 1 | 109 ± 32* | 0 | 0 | |||||
| IL-3 | 6 ± 1 | 16 ± 5 | 10 ± 3 | 22 ± 11 | 2 ± 1 | 6 ± 3 | |||||
| SCF + IL-3 + EPO | 9 ± 4 | 20 ± 8 | 11 ± 6 | 15 ± 9 | 1 ± 2 | 23 ± 8 | |||||
. | Mean no. colonies, ± SD . | . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Stimulus . | Blast . | Granulocyte . | Granulocyte-macrophage . | Macrophage . | Eosinophil . | Megakaryocyte . | |||||
| Wild-type, 2 wk; n = 3 | |||||||||||
| GM-CSF | 0 | 15 ± 4 | 7 ± 4 | 17 ± 4 | 2 ± 1 | 0 | |||||
| M-CSF | 0 | 0.7 ± 0.6 | 3 ± 1 | 50 ± 17* | 0 | 0 | |||||
| IL-3 | 8 ± 2 | 12 ± 4 | 8 ± 3 | 11 ± 12 | 2 ± 2 | 5 ± 2 | |||||
| SCF + IL-3 + EPO | 8 ± 3 | 18 ± 6 | 12 ± 11 | 11 ± 12 | 2 ± 2 | 24 ± 5 | |||||
| MYC17, 2 wk; n = 3 | |||||||||||
| GM-CSF | 0 | 10 ± 2 | 4 ± 0 | 19 ± 8 | 2 ± 1 | 0 | |||||
| M-CSF | 0 | 0 | 2 ± 2 | 158 ± 48* | 0 | 0 | |||||
| IL-3 | 5 ± 2 | 9 ± 3 | 3 ± 3 | 13 ± 6 | 3 ± 3 | 6 ± 2 | |||||
| SCF + IL-3 + EPO | 8 ± 3 | 7 ± 3 | 3 ± 2 | 13 ± 15 | 0.7 ± 1.2 | 11 ± 4 | |||||
| Wild-type, 8 wk; n = 4 | |||||||||||
| GM-CSF | 0 | 16 ± 5 | 5 ± 2 | 31 ± 3 | 4 ± 2 | 0 | |||||
| M-CSF | 0 | 3 ± 1 | 6 ± 4 | 49 ± 8* | 0 | 0 | |||||
| IL-3 | 7 ± 4 | 19 ± 6 | 11 ± 3 | 15 ± 3 | 2 ± 1 | 7 ± 4 | |||||
| SCF + IL-3 + EPO | 11 ± 6 | 21 ± 2 | 13 ± 3 | 17 ± 3 | 2 ± 1 | 28 ± 4 | |||||
| MYC10, 8 wk; n = 4 | |||||||||||
| GM-CSF | 0 | 19 ± 6 | 5 ± 2 | 29 ± 15 | 3 ± 3 | 0 | |||||
| M-CSF | 0 | 3 ± 1 | 2 ± 1 | 109 ± 32* | 0 | 0 | |||||
| IL-3 | 6 ± 1 | 16 ± 5 | 10 ± 3 | 22 ± 11 | 2 ± 1 | 6 ± 3 | |||||
| SCF + IL-3 + EPO | 9 ± 4 | 20 ± 8 | 11 ± 6 | 15 ± 9 | 1 ± 2 | 23 ± 8 | |||||
Bone marrow cells from healthy young mice of the age and genotype indicated were cultured in agar and stimulated with cytokines (GM-CSF, G-CSF, M-CSF, IL-3, EPO, SCF, G-CSF + SCF, TPO, FIt3L + LIF, SCF + IL-3 + EPO, IFNγ, IL-6) for 7 days. The resultant colonies were stained, examined for composition, and counted. The results with 4 different stimuli are shown as examples.
Significant difference from wild-type controls was that the frequency of M-CSF-dependent macrophage colonies was increased for both MYC17 and MYC10 mice.