Altered composition of the spleen in healthy young MYC10 and MYC10hom mice
. | Cell no. per spleen, × 106* . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|
| Cell type . | Wild-type . | MYC10 . | Ratio†(P) . | MYC10hom . | Ratio†(P) . | ||||
| Total | 180 ± 20 | 240 ± 20 | 1.3 (.03) | 310 ± 40 | 1.7 (.01) | ||||
| Pre-B | 2.9 ± 0.3 | 4.1 ± 0.5 | 1.4 (.06) | 7.2 ± 0.1 | 2.5 (< .01) | ||||
| B | 110 ± 10 | 140 ± 10 | 1.3 (.03) | 190 ± 20 | 1.7 (.01) | ||||
| CD4+ CD8- T | 31 ± 4 | 42 ± 2 | 1.4 (.03) | 44 ± 2 | 1.4 (.02) | ||||
| CD4- CD8+ T | 18 ± 2 | 24 ± 2 | 1.3 (.06) | 24 ± 2 | 1.3 (.07) | ||||
| Erythroid | 0.9 ± 0.3 | 2.8 ± 0.6 | 3.1 (.02) | 9.5 ± 3.2 | 10.6 (.03) | ||||
| Monocyte | 5.2 ± 0.8 | 6.4 ± 0.4 | 1.2 (.13) | 8.6 ± 1.1 | 1.7 (.03) | ||||
| Granulocyte | 4.0 ± 0.2 | 4.5 ± 0.6 | 1.1 (.23) | 10 ± 3 | 2.5 (.04) | ||||
. | Cell no. per spleen, × 106* . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|
| Cell type . | Wild-type . | MYC10 . | Ratio†(P) . | MYC10hom . | Ratio†(P) . | ||||
| Total | 180 ± 20 | 240 ± 20 | 1.3 (.03) | 310 ± 40 | 1.7 (.01) | ||||
| Pre-B | 2.9 ± 0.3 | 4.1 ± 0.5 | 1.4 (.06) | 7.2 ± 0.1 | 2.5 (< .01) | ||||
| B | 110 ± 10 | 140 ± 10 | 1.3 (.03) | 190 ± 20 | 1.7 (.01) | ||||
| CD4+ CD8- T | 31 ± 4 | 42 ± 2 | 1.4 (.03) | 44 ± 2 | 1.4 (.02) | ||||
| CD4- CD8+ T | 18 ± 2 | 24 ± 2 | 1.3 (.06) | 24 ± 2 | 1.3 (.07) | ||||
| Erythroid | 0.9 ± 0.3 | 2.8 ± 0.6 | 3.1 (.02) | 9.5 ± 3.2 | 10.6 (.03) | ||||
| Monocyte | 5.2 ± 0.8 | 6.4 ± 0.4 | 1.2 (.13) | 8.6 ± 1.1 | 1.7 (.03) | ||||
| Granulocyte | 4.0 ± 0.2 | 4.5 ± 0.6 | 1.1 (.23) | 10 ± 3 | 2.5 (.04) | ||||
Eight-week-old mice were analyzed. Total nucleated counts were determined with a Coulter counter and subpopulations by flow cytometry. Cell types examined were pre-B (B220+ IgM-), B (B220+ IgM+), erythroid (Ter119+), granulocyte (Gr1+ Mac1+), monocyte (Gr1- Mac1+), and T-cell subpopulations (based on CD4/CD8 expression). P for a t test.
Mean ± SEM; n = 3.
Ratio of MYC10 or MYC10hom to wild-type control.