Quantitative real-time PCR for Fas, FasL, and FasB mRNAs in chronic erythropoietic stress
. | Fas . | . | FasL . | . | FasB . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| Mouse strain* . | ΔΔCT† . | Fold decrease in mRNA‡ . | ΔΔCT† . | Fold decrease in mRNA‡ . | ΔΔCT† . | Fold decrease in mRNA‡ . | |||
| ts-VHL−/− | |||||||||
| Experiment 1 | 1.12 ± 0.29 | — | 0.99 ± 0.42 | — | 1.12 ± 0.80 | — | |||
| Experiment 2 | 2.61 ± 0.09 | — | 2.52 ± 0.17 | — | 2.64 ± 0.86 | — | |||
| Experiment 3 | 2.05 ± 0.17 | — | 1.44 ± 0.16 | — | 2.51 ± 0.50 | — | |||
| Experiment 4 | 1.00 ± 0.13 | — | 1.19 ± 0.07 | — | 0.49 ± 0.30 | — | |||
| Mean | 1.70 ± 0.38 | 3.2 | 1.54 ± 0.34 | 2.9 | 1.69 ± 0.52 | 3.2 | |||
| β-thal | |||||||||
| Experiment 1 | 1.75 ± 0.28 | — | 0.16 ± 0.66 | — | 2.29 ± 0.90 | — | |||
| Experiment 2 | 3.56 ± 0.26 | — | 1.9 ± 0.24 | — | 4.3 ± 0.09 | — | |||
| Experiment 3 | 2.35 ± 0.07 | — | (−0.5) ± 0.47 | — | 2.14 ± 0.08 | — | |||
| Mean | 2.55 ± 0.53 | 5.9 | 0.52 ± 0.7 | 1.4 | 2.92 ± 0.70 | 7.6 | |||
. | Fas . | . | FasL . | . | FasB . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| Mouse strain* . | ΔΔCT† . | Fold decrease in mRNA‡ . | ΔΔCT† . | Fold decrease in mRNA‡ . | ΔΔCT† . | Fold decrease in mRNA‡ . | |||
| ts-VHL−/− | |||||||||
| Experiment 1 | 1.12 ± 0.29 | — | 0.99 ± 0.42 | — | 1.12 ± 0.80 | — | |||
| Experiment 2 | 2.61 ± 0.09 | — | 2.52 ± 0.17 | — | 2.64 ± 0.86 | — | |||
| Experiment 3 | 2.05 ± 0.17 | — | 1.44 ± 0.16 | — | 2.51 ± 0.50 | — | |||
| Experiment 4 | 1.00 ± 0.13 | — | 1.19 ± 0.07 | — | 0.49 ± 0.30 | — | |||
| Mean | 1.70 ± 0.38 | 3.2 | 1.54 ± 0.34 | 2.9 | 1.69 ± 0.52 | 3.2 | |||
| β-thal | |||||||||
| Experiment 1 | 1.75 ± 0.28 | — | 0.16 ± 0.66 | — | 2.29 ± 0.90 | — | |||
| Experiment 2 | 3.56 ± 0.26 | — | 1.9 ± 0.24 | — | 4.3 ± 0.09 | — | |||
| Experiment 3 | 2.35 ± 0.07 | — | (−0.5) ± 0.47 | — | 2.14 ± 0.08 | — | |||
| Mean | 2.55 ± 0.53 | 5.9 | 0.52 ± 0.7 | 1.4 | 2.92 ± 0.70 | 7.6 | |||
RNA prepared and real-time PCR carried out independently for each experiment, from Ery.A sorted from at least one β-thal or ts-VHL −/− mouse and one matched littermate.
Mean ± SD of ΔΔCT from triplicate samples for each experiment. ΔΔCT is the difference in cycle number for the indicated mRNAs, between template RNA from β-thal or ts-VHL −/− mouse and its matched littermate, relative to β-actin mRNA in each sample.
Calculated as 2 to the power of the mean ΔΔCT for all experiments.