Down-regulation of splenic erythroblast cell-surface Fas/FasL in ts-VHL−/− mice
. | Subset frequency, % of Ter 119+ cells . | . | . | Fas-positive cells, % . | . | . | FasL-positive cells, % . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | Control*; n = 6 . | ts-VHL −/−; n = 5 . | P† . | Control*; n = 6 . | ts-VHL −/−; n = 5 . | P† . | Control*; n = 6 . | ts-VHL −/−; n = 5 . | P† . | ||||||
| Spleen | |||||||||||||||
| ProE | 0.6 ± 0.2 | 1.5 ± 0.3 | — | 38 ± 9 | 9 ± 3 | .023 | 66 ± 4 | 36 ± 9 | .025 | ||||||
| Ery.A | 24 ± 5 | 36 ± 3 | .044 | 48 ± 6 | 26 ± 3 | .011 | 79 ± 2 | 60 ± 5 | .018 | ||||||
| Ery.B | 24 ± 4 | 39 ± 3 | .012 | 12 ± 1 | 3 ± 0.4 | < .001 | 37 ± 4 | 12 ± 4 | .002 | ||||||
| Ery.C | 52 ± 8 | 24 ± 4 | .045 | 0.6 ± 0.1 | 0.3 ± 0.1 | — | 1 ± 0.2 | 0.6 ± 0.2 | — | ||||||
| Non-Ery | NA | NA | — | 47 ± 4 | 47 ± 6 | — | 10 ± 0.2 | 9 ± 1 | — | ||||||
| Bone marrow | |||||||||||||||
| ProE | 1.9 ± 0.3 | 2.0 ± 0.4 | — | 21 ± 3 | 13 ± 3 | — | 35 ± 4 | 28 ± 5 | — | ||||||
| Ery.A | 35 ± 4 | 35 ± 5 | — | 21 ± 2 | 17 ± 4 | — | 51 ± 3 | 41 ± 4 | — | ||||||
| Ery.B | 34 ± 2 | 25 ± 1 | .001 | 2 ± 0.5 | 1 ± 0.2 | — | 9 ± 2 | 6 ± 2 | — | ||||||
| Ery.C | 29 ± 4 | 38 ± 6 | — | 0.3 ± 0.05 | 0.3 ± 0.1 | — | 0.6 ± 0.1 | 0.3 ± 0.1 | — | ||||||
| Non-Ery | NA | NA | — | 51 ± 4 | 50 ± 3 | — | 15 ± 4 | 18 ± 5 | — | ||||||
. | Subset frequency, % of Ter 119+ cells . | . | . | Fas-positive cells, % . | . | . | FasL-positive cells, % . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | Control*; n = 6 . | ts-VHL −/−; n = 5 . | P† . | Control*; n = 6 . | ts-VHL −/−; n = 5 . | P† . | Control*; n = 6 . | ts-VHL −/−; n = 5 . | P† . | ||||||
| Spleen | |||||||||||||||
| ProE | 0.6 ± 0.2 | 1.5 ± 0.3 | — | 38 ± 9 | 9 ± 3 | .023 | 66 ± 4 | 36 ± 9 | .025 | ||||||
| Ery.A | 24 ± 5 | 36 ± 3 | .044 | 48 ± 6 | 26 ± 3 | .011 | 79 ± 2 | 60 ± 5 | .018 | ||||||
| Ery.B | 24 ± 4 | 39 ± 3 | .012 | 12 ± 1 | 3 ± 0.4 | < .001 | 37 ± 4 | 12 ± 4 | .002 | ||||||
| Ery.C | 52 ± 8 | 24 ± 4 | .045 | 0.6 ± 0.1 | 0.3 ± 0.1 | — | 1 ± 0.2 | 0.6 ± 0.2 | — | ||||||
| Non-Ery | NA | NA | — | 47 ± 4 | 47 ± 6 | — | 10 ± 0.2 | 9 ± 1 | — | ||||||
| Bone marrow | |||||||||||||||
| ProE | 1.9 ± 0.3 | 2.0 ± 0.4 | — | 21 ± 3 | 13 ± 3 | — | 35 ± 4 | 28 ± 5 | — | ||||||
| Ery.A | 35 ± 4 | 35 ± 5 | — | 21 ± 2 | 17 ± 4 | — | 51 ± 3 | 41 ± 4 | — | ||||||
| Ery.B | 34 ± 2 | 25 ± 1 | .001 | 2 ± 0.5 | 1 ± 0.2 | — | 9 ± 2 | 6 ± 2 | — | ||||||
| Ery.C | 29 ± 4 | 38 ± 6 | — | 0.3 ± 0.05 | 0.3 ± 0.1 | — | 0.6 ± 0.1 | 0.3 ± 0.1 | — | ||||||
| Non-Ery | NA | NA | — | 51 ± 4 | 50 ± 3 | — | 15 ± 4 | 18 ± 5 | — | ||||||