Detection of bacterial virulence factors and platelet activation markers on platelets from HUS patients by immunofluorescence
. | Patient and strain characteristics . | . | . | Markers on platelets . | . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Patient no. . | Diagnosis . | E coli serotype* . | Fecal stx type† . | O157LPS . | Stx1 . | Stx2 . | CD62 . | Fibrinogen . | ||||||
| 1 | HUS | O157 | stx2 | + | + | − | − | + | ||||||
| 2 | HUS | O157§ | stx2 | + | + | − | − | + | ||||||
| 3 | HUS | O157∥ | stx2 | + | + | + | NA | + | ||||||
| 4 | HUS | O157∥ | stx2 | + | + | − | NA | − | ||||||
| 5 | HUS | O157 | stx2 | − | − | − | + | + | ||||||
| 6 | HC‡ | O157∥ | stx2 | + | − | + | − | + | ||||||
| 7 | HUS | O111¶ | NA | − | + | − | + | + | ||||||
| 8 | HUS | O157¶ | NA | + | + | − | − | − | ||||||
| 9 | HUS | O157¶ | NA | + | NA | NA | − | + | ||||||
| 10 | HUS | O157 | stx2 | + | + | + | − | + | ||||||
| 11 | HUS | O157∥ | stx2 | + | + | + | − | + | ||||||
| 12 | HUS | O157∥¶ | NA | + | + | + | − | + | ||||||
| 13 | HUS | O157¶ | NA | + | + | + | − | + | ||||||
| 14 | HUS | O157 | stx2 | − | − | + | − | + | ||||||
| 15 | Pre-HUS | O157 | stx1,stx2 | + | + | − | + | − | ||||||
| 16 | Pre-HUS | O157 | stx2 | + | + | − | + | + | ||||||
| 16 | HUS | O157 | stx2 | + | + | − | − | + | ||||||
. | Patient and strain characteristics . | . | . | Markers on platelets . | . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Patient no. . | Diagnosis . | E coli serotype* . | Fecal stx type† . | O157LPS . | Stx1 . | Stx2 . | CD62 . | Fibrinogen . | ||||||
| 1 | HUS | O157 | stx2 | + | + | − | − | + | ||||||
| 2 | HUS | O157§ | stx2 | + | + | − | − | + | ||||||
| 3 | HUS | O157∥ | stx2 | + | + | + | NA | + | ||||||
| 4 | HUS | O157∥ | stx2 | + | + | − | NA | − | ||||||
| 5 | HUS | O157 | stx2 | − | − | − | + | + | ||||||
| 6 | HC‡ | O157∥ | stx2 | + | − | + | − | + | ||||||
| 7 | HUS | O111¶ | NA | − | + | − | + | + | ||||||
| 8 | HUS | O157¶ | NA | + | + | − | − | − | ||||||
| 9 | HUS | O157¶ | NA | + | NA | NA | − | + | ||||||
| 10 | HUS | O157 | stx2 | + | + | + | − | + | ||||||
| 11 | HUS | O157∥ | stx2 | + | + | + | − | + | ||||||
| 12 | HUS | O157∥¶ | NA | + | + | + | − | + | ||||||
| 13 | HUS | O157¶ | NA | + | + | + | − | + | ||||||
| 14 | HUS | O157 | stx2 | − | − | + | − | + | ||||||
| 15 | Pre-HUS | O157 | stx1,stx2 | + | + | − | + | − | ||||||
| 16 | Pre-HUS | O157 | stx2 | + | + | − | + | + | ||||||
| 16 | HUS | O157 | stx2 | + | + | − | − | + | ||||||
Patients 1-14 were treated at the Department of Pediatrics, Lund, Sweden; patients 15 and 16, at CHRMC, Seattle, WA.
NA indicates not available; HC, hemorrhagic colitis.
E. coli serotype was determined by serotyping of a fecal isolate or by serum antibodies to specific LPS serotypes.26
Fecal Shiga toxin type was determined by PCR.
Incomplete HUS.
Fecal PCR27 results were positive for stx and eae, but a bacterial strain was not isolated. E. coli serotype was determined by ELISA detection of anti-O157LPS.
LPS was not detected on the platelets of these patients after recovery.
Feces were unavailable or fecal PCR was negative, and the E. coli serotype was determined by ELISA for the detection of anti-LPS.